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Manufactured by BioVendor

CTRP1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:CTRP1
  • Species:Human
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Cat. No. Size Price


Order in advance RD191153100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-Citrate

Sample Requirements

8 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.13–100 ng/ml

Limit of Detection

0.016 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 2.7%

Inter-assay (Run-to-Run)

n = 5; CV = 8.5%

Spiking Recovery

94,90%

Dilution Linearity

100,20%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Non-detectable
  • monkey Yes
  • mouse Yes
  • human Yes
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures CTRP1 in serum and plasma citrate
  • Assay format is 96 wells
  • Quality Controls are human serum based
  • Standard is recombinant protein
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Summary

CTRP1 (Complement C1q tumor necrosis factor-related protein 1) is a member of the CTRP superfamily and is localized to human chromosome 17. It is a 32 kDa secreted glycoprotein comprised of 281 amino acids encoded by the human gene C1QTN1. This protein is a highly conserved paralogue of adiponectin, containing a cluster of collagenlike repeats, a C-terminal globular ‘C1q-like’ domain and an N-terminal signal peptide
sequence followed by a variable region and hence is predicted to be secreted protein. CTRP1 is primarily expressed from cells in the stroma vascular fraction of adipose tissue and is also specifically expressed in the zona glomerulosa of the adrenal cortex and in vascular wall tissue. This protein is ubiquitous in most regions of the human brain and is particularly abundant in the spinal cord, but the pattern of expression and functions of CTRP1 in brain are completely unknown. Expression of CTRP1 (like adiponectin) is induced by PPARg (peroxisome-proliferator-activated receptor γ). The LPS -induced increase in CTRP1 gene expression is found to be mediated by TNF-alpha and IL-1beta. Sequence homology inferred that the CTRP1 cytosolic domain is folded as a collagen-like helix followed by a globular domain. The interaction of the globular domain with the V2R was confirmed by pull-down experiments indicating that this structural motif can also interact with cytosolic proteins. Obesity and the metabolic syndrome are frequently associated with elevated levels of aldosterone and the level of CTRP1 is increased in the blood of hypertensive patients, as aldosterone production was stimulated by CTRP1. It is well known that obesity is the leading cause of hypertension. CTRP1 is stimulating aldosterone production through induction of CYP11B2 gene expression. Therefore, CTRP1 could be a molecular link between obesity and hypertension.
Areas of investigation: Energy metabolism and body weight regulation, Metabolic syndrome.

Product References (9)

References

  • Bai B, Ban B, Liu Z, Zhang MM, Tan BK, Chen J. Circulating C1qcomplement/TNF-related protein (CTRP) 1, CTRP9, CTRP12 and CTRP13 concentrations in Type 2 diabetes mellitus: In vivo regulation by glucose. PLoS One. 2017 Feb16;12(2):e0172271. doi: 10.1371/journal.pone.0172271. eCollection 2017. PubMedPMID: 28207876; PubMed Central PMCID: PMC5313218. See more on PubMed
  • Barbieri D, Goicoechea M, Sánchez-Niño MD, Ortiz A, Verde E, Verdalles U,Pérez de José A, Delgado A, Hurtado E, Sánchez-Cámara L, Lopez-Lazareno N,García-Prieto A, Luño J. Obesity and chronic kidney disease progression-the role of a new adipocytokine: C1q/tumour necrosis factor-related protein-1. Clin KidneyJ. 2018 Oct 11;12(3):420-426. doi: 10.1093/ckj/sfy095. eCollection 2019 Jun.PubMed PMID: 31198543; PubMed Central PMCID: PMC6543966. See more on PubMed
  • Han S, Kim JD, Lee S, Jeong AL, Park JS, Yong HJ, Boldbaatar A, Ka HI, RheeEJ, Lee WY, Yang Y. Circulating CTRP1 Levels in Type 2 Diabetes and TheirAssociation with FGF21. Int J Endocrinol. 2016;2016:5479627. doi:10.1155/2016/5479627. Epub 2016 May 23. PubMed PMID: 27313611; PubMed CentralPMCID: PMC4893584. See more on PubMed
  • Chalupova L, Zakovska A, Adamcova K. Development of a novel enzyme-linkedimmunosorbent assay (ELISA) for measurement of serum CTRP1: a pilot study:measurement of serum CTRP1 in healthy donors and patients with metabolicsyndrome. Clin Biochem. 2013 Jan;46(1-2):73-8. doi:10.1016/j.clinbiochem.2012.09.006. Epub 2012 Sep 19. PubMed PMID: 23000311. See more on PubMed
  • Pan X, Lu T, Wu F, Jin L, Zhang Y, Shi L, Li X, Lin Z. Circulatingcomplement-C1q TNF-related protein 1 levels are increased in patients with type 2diabetes and are associated with insulin sensitivity in Chinese subjects. PLoSOne. 2014 May 14;9(5):e94478. doi: 10.1371/journal.pone.0094478. eCollection2014. PubMed PMID: 24827430; PubMed Central PMCID: PMC4037153. See more on PubMed
  • Rodriguez S, Lei X, Petersen PS, Tan SY, Little HC, Wong GW. Loss of CTRP1disrupts glucose and lipid homeostasis. Am J Physiol Endocrinol Metab. 2016 Oct1;311(4):E678-E697. doi: 10.1152/ajpendo.00087.2016. Epub 2016 Aug 23. PubMedPMID: 27555298; PubMed Central PMCID: PMC5241556. See more on PubMed
  • Shabani P, Naeimi Khaledi H, Beigy M, Emamgholipour S, Parvaz E, Poustchi H,Doosti M. Circulating level of CTRP1 in patients with nonalcoholic fatty liverdisease (NAFLD): is it through insulin resistance? PLoS One. 2015 Mar13;10(3):e0118650. doi: 10.1371/journal.pone.0118650. eCollection 2015. PubMedPMID: 25767880; PubMed Central PMCID: PMC4358971. See more on PubMed
  • Xin Y, Lyu X, Wang C, Fu Y, Zhang S, Tian C, Li Q, Zhang D. Elevatedcirculating levels of CTRP1, a novel adipokine, in diabetic patients. Endocr J.2014;61(9):841-7. Epub 2014 Jun 24. PubMed PMID: 24965225. See more on PubMed
  • Yagmur E, Buergerhausen D, Koek GH, Weiskirchen R, Trautwein C, Koch A, Tacke F. Elevated CTRP1 Plasma Concentration Is Associated with Sepsis and Pre-ExistingType 2 Diabetes Mellitus in Critically Ill Patients. J Clin Med. 2019 May11;8(5). pii: E661. doi: 10.3390/jcm8050661. PubMed PMID: 31083558; PubMedCentral PMCID: PMC6572622. See more on PubMed
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