Total 255 AA. MW: 28,4 kDa (calculated). UniProtKB acc.no. P60827. N-Terminal His-tag, 10 extra AA (highlighted).
Amino Acid Sequence
14% SDS-PAGE separation of Human CTRP8
1. reduced and heated sample, 5μg / lane
2. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
3. non-reduced and non-heated sample, 5μg / lane
< 0.1 EU/μg
0.4 – 1.0 mg/ml in 0.03M acetate buffer, pH=4.0 – filtered (0.4 μm), frozen
Defrost at ambient temperature. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.