Total 68 AA. MW: 8,0 kDa (calculated). UniProtKB acc.no# P48061 2 (24–89) Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by SDS PAGE: >97 %
EC50 = 0.31nM determined by Calcium Flux with human CXCR4 in U937 cells EC50 = 0.17nM determined by Migration with U937 cells SPA Binding assay of CXCL12/SDF-1α to CXCR7 (IC50 = 14pM) and CXCR4 (IC50 = 2nM)
<0.01 EU /1µg
Add sterile water to prepare a working stock solution of approximately 0.1 mg/ml and let the lyophilized pellet dissolve completely.
Western blotting, ELISA, Cell culture and/or animal studies, Biologically active protein
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –20 to –70 °C. Lyophilized protein remains stable until the expiry date when stored at-20 to –70 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -20 to –70 °C, the protein is stable for 3 months at –20 to –70 °C under sterile conditions after reconstitution. Reconstituted protein can be stored at 4 °C for 1 month under sterile conditions after reconstitution
Quality Control Test
Quantification is done by A280, using the calculated extinction coefficient (8730 M-1 cm-1).
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.
This product is intended for research use only.