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Manufactured by BioVendor

Cyclophilin-40 Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Peptidyl-prolyl cis-trans isomerase D, PPIase D, 40 kDa peptidyl-prolyl cis-trans isomerase, CYP-40, Cyclophilin-related protein, Estrogen receptor-binding cyclophilin, Rotamase D, PPID, CYPD
  • Species:Human
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Cat. No. Size Price


RD172362100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 378 AA. MW: 41.9 kDa (calculated). UniProtKB acc.no. Q08752 (Ser2-Ala370). N-terminal His-tag (9 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHASHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95%

SDS-PAGE Gel

14 % SDS-PAGE separation of Human Cyclophlin 40 (E.coli):
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/μg

Formulation

Filtered (0.4 μm) and lyophilized in phosphate buffered saline.

Reconstitution

Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Neural tissue markers, Oncology

Summary

Cyclophilin 40 (CyP40, rotamase D, PPID) was first described as a cyclosporine A (CsA) binding protein and part of the inactivated estrogen receptor complex. CyP 40 functions include contributing to protein folding, ligand binding, and nuclear localization of glucocorticoid, estrogen and progesterone receptors. One of the important roles of CyP 40 is to help with the assembling of heat shock protein Hsp90 in chaperone protein-folding machinery.
Many functions of Hsp90 are dependent on its association with co-chaperone proteins. Co-chaperones mediate various aspects of Hsp90 function, including the association of Hsp90 with client proteins and the regulation of Hsp90 ATPase activity. CyP 40 is a member of the immunophilin family of Hsp90 co-chaperones.This family is characterized by its association with Hsp90-steroid hormone receptor complexes containing client proteins such as the glucocorticoid, estrogen, progesterone, and androgen receptors. Immunophilin co-chaperones are important in cancer, as CyP40 has been shown to promote the proliferation of androgen-dependent and androgen-independent prostate cancer cell lines.
A recent study showed that CyP40 mRNA was over-expressed in breast cancer tissues as compared with normal breast control tissues, and CyP 40 mRNA was ubiquitously expressed in 10 breast cancer cell lines. CyP40 also plays an important role in chronic hepatitis virus (HCV) replication. Baker et al. demonstrated that CyP 40 is capable of disaggregating amyloid fibrils in vitro and data from this study implicate that CyP 40 is a potential therapeutic intervention for tauopathies and other amyloidogenic disorders.

References to Summary

References to Cyclophilin 40

  • Jandova J, Janda J and Sligh JE: Cyclophilin 40 alters UVA-induced apoptosis and mitochondrial ROS generation in keratinocytes. Exp Cell Res 2013; 319 (5):750-760
  • Naoumov NV: Cyclophilin inhibition as potential therapy for liver diseases. Journal of Hepatology 2014; 61:1166-1174
  • Park MS, Chu F, Xie J, Wang Y, Bhattacharya P, Chan WK: Identification of cyclophilin - 40-interacting proteins reveals potential cellular function of cyclohilin - 40. Analytical Biochemistry 2011; 410:257-265
  • Pearson JD, Mohammed Z, Bacani JTC, Lai R and Ingham RJ: The heat shock protein-90 co-chaperone, Cyclophilin 40, promotes ALK-positive, anaplastic large cell lymphoma viability and its expression is regulated by the NPM-ALK oncoprotein. BMC Cancer 2012; 12:229 1-14
  • Ward BK, Mark PJ, Ingram DM, Minchin RF, Ratajczak T: Expression of the estrogen receptor associated immunophilins, cyclophilin 40 and FKBP52, in breast cancer. Breast Cancer Rest Treat 1999; 58:267-280
  • Anderson LJ, Lin K, Compton T and Wiedmann B: Inhibition of cyclophilins alters lipid trafficking and blocks hepatis C virus secretion. Virology Journal 2011; 8:329 1-13
  • Baker JD, Shelton LB, Yheng D, Favretto F, Nordhues BA, Darling A, Sullivan LE, Sun Z, Solanki PK, Martin MD, Suntharalingam A, Sabbagh JJ, Becker S, Mandelkow E, Uversky VN, Zweckstetter M, Dickey CHA, Koren III J, Blair LJ: Human cyclophilin 40 unravels neurotoxic amyloids. PLoS Biol 2017; 15(6): 1-22
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