Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Urine
Sample Requirements
5 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.31–10 ng/ml
Limit of Detection
0.005 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 6.3%
Inter-assay (Run-to-Run)
n = 5; CV = 5.7%
Spiking Recovery
103,70%
Dilution Linearity
91,40%
Crossreactivity
- bovine Non-detectable
- cat Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Non-detectable
- dog Yes
- monkey Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- human Non-detectable
- chicken Not tested
Features
- It is intended for research use only
- The total assay time is less than 3 hours
- The kit measures total cystatin C in canine serum and urine
- Assay format is 96 wells
- Quality Controls are animal serum based. No human sera are used
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Neural tissue markers, Renal disease, Animal studies
Summary
Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
