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You are here:   Home Products and Services Immunoassays Cystatin C Canine ELISA
Manufactured by BioVendor

Cystatin C Canine ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Post G-globulin, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3
  • Species:Canine
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Cat. No. Size Price

RD491009100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine

Sample Requirements

5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.31–10 ng/ml

Limit of Detection

0.005 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.3%

Inter-assay (Run-to-Run)

n = 5; CV = 5.7%

Spiking Recovery

103,70%

Dilutation Linearity

91,40%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • dog Yes
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • human Non-detectable
  • chicken Not tested
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures total cystatin C in canine serum and urine
  • Assay format is 96 wells
  • Quality Controls are animal serum based. No human sera are used
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Animal studies, Neural tissue markers, Renal disease

Summary

Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's di­sease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.

References to Product

References

  • Martineau AS, Leray V, Lepoudere A, Blanchard G, Bensalem J, Gaudout D, Ouguerram K, Nguyen P, ORCID: http://orcid.org/0000-0002-4263-025X. A mixed grape and blueberry extract is safe for dogs to consume. BMC Vet Res. 2016;12 (1):162
  • Tvarijonaviciute A, Ceron JJ, Holden SL, Biourge V, Morris PJ, German AJ. Effect of weight loss in obese dogs on indicators of renal function or disease. J Vet Intern Med. 2013 Jan-Feb;27 (1):31-8
References to Summary

References to Cystatin C

  • Bokenkamp A, Domanetzki M, Zinck R, Schumann G, Byrd D, Brodehl J. Cystatin C--a new marker of glomerular filtration rate in children independent of age and height. Pediatrics. 1998 May;101 (5):875-81
  • Delanaye P, Cavalier E, Krzesinski JM. Cystatin C, renal function, and cardiovascular risk. Ann Intern Med. 2008 Feb 19;148 (4):323
  • Deng A, Irizarry MC, Nitsch RM, Growdon JH, Rebeck GW. Elevation of cystatin C in susceptible neurons in Alzheimer's disease. Am J Pathol. 2001 Sep;159 (3):1061-8
  • Dharnidharka VR, Kwon C, Stevens G. Serum cystatin C is superior to serum creatinine as a marker of kidney function: a meta-analysis. Am J Kidney Dis. 2002 Aug;40 (2):221-6
  • Ekiel I, Abrahamson M, Fulton DB, Lindahl P, Storer AC, Levadoux W, Lafrance M, Labelle S, Pomerleau Y, Groleau D, LeSauteur L, Gehring K. NMR structural studies of human cystatin C dimers and monomers. J Mol Biol. 1997 Aug 15;271 (2):266-77
  • Luc G, Bard JM, Lesueur C, Arveiler D, Evans A, Amouyel P, Ferrieres J, Juhan-Vague I, Fruchart JC, Ducimetiere P. Plasma cystatin-C and development of coronary heart disease: The PRIME Study. Atherosclerosis. 2006 Apr;185 (2):375-80
  • Macisaac RJ, Tsalamandris C, Thomas MC, Premaratne E, Panagiotopoulos S, Smith TJ, Poon A, Jenkins MA, Ratnaike SI, Power DA, Jerums G. Estimating glomerular filtration rate in diabetes: a comparison of cystatin-C- and creatinine-based methods. Diabetologia. 2006 Jul;49 (7):1686-9
  • Mojiminiyi OA, Abdella N. Evaluation of cystatin C and beta-2 microglobulin as markers of renal function in patients with type 2 diabetes mellitus. J Diabetes Complications. 2003 May-Jun;17 (3):160-8
  • Moran A, Katz R, Smith NL, Fried LF, Sarnak MJ, Seliger SL, Psaty B, Siscovick DS, Gottdiener JS, Shlipak MG. Cystatin C concentration as a predictor of systolic and diastolic heart failure. J Card Fail. 2008 Feb;14 (1):19-26
  • Muntner P, Mann D, Winston J, Bansilal S, Farkouh ME. Serum cystatin C and increased coronary heart disease prevalence in US adults without chronic kidney disease. Am J Cardiol. 2008 Jul 1;102 (1):54-7
  • Muntner P, Winston J, Uribarri J, Mann D, Fox CS. Overweight, obesity, and elevated serum cystatin C levels in adults in the United States. Am J Med. 2008 Apr;121 (4):341-8
  • Mussap M, Plebani M. Biochemistry and clinical role of human cystatin C. Crit Rev Clin Lab Sci. 2004;41 (5-6):467-550
  • Nakashima I, Fujinoki M, Fujihara K, Kawamura T, Nishimura T, Nakamura M, Itoyama Y. Alteration of cystatin C in the cerebrospinal fluid of multiple sclerosis. Ann Neurol. 2007 Aug;62 (2):197-200; discussion 205
  • Ortiz F, Harmoinen A, Paavonen T, Koskinen P, Gronhagen-Riska C, Honkanen E. Is Cystatin C more sensitive than creatinine in detecting early chronic allograft nephropathy?. Clin Nephrol. 2008 Jul;70 (1):18-25
  • Parikh NI, Hwang SJ, Yang Q, Larson MG, Guo CY, Robins SJ, Sutherland P, Benjamin EJ, Levy D, Fox CS. Clinical correlates and heritability of cystatin C (from the Framingham Offspring Study). Am J Cardiol. 2008 Nov 1;102 (9):1194-8
  • Perlemoine C, Beauvieux MC, Rigalleau V, Baillet L, Barthes N, Derache P, Gin H. Interest of cystatin C in screening diabetic patients for early impairment of renal function. Metabolism. 2003 Oct;52 (10):1258-64
  • Premaratne E, MacIsaac RJ, Finch S, Panagiotopoulos S, Ekinci E, Jerums G. Serial measurements of cystatin C are more accurate than creatinine-based methods in detecting declining renal function in type 1 diabetes. Diabetes Care. 2008 May;31 (5):971-3
  • Risch L, Blumberg A, Huber A. Rapid and accurate assessment of glomerular filtration rate in patients with renal transplants using serum cystatin C. Nephrol Dial Transplant. 1999 Aug;14 (8):1991-6
  • Samouilidou EC, Grapsa E. Relationship of serum cystatin C with C-reactive protein and apolipoprotein A1 in patients on hemodialysis. Ren Fail. 2008;30 (7):711-5
  • Servais A, Giral P, Bernard M, Bruckert E, Deray G, Isnard Bagnis C. Is serum cystatin-C a reliable marker for metaboli
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