Native protein isolated from human urine.
Purity as determined by densitometric image analysis: >96%
12% SDS-PAGE separation of Human Cystatin C
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane
< 1.0 EU/ug
Filtered (0,4 μm)) and lyophilised from 0.6 mg/mL in 20mM NH4HCO3 with traces of buffer salts.
Add 20mM NH4HCO3 to prepare a working stock solution of approximately 0.5 mg/mL. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only. All starting material used for purification was tested and found negative for HIV 1 and 2 antibodies, Hepatitis B surface antigen and HCV antibodies.