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Manufactured by BioVendor

Cysteine-Rich Secretory Protein 1 Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:CRISP-1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog, AEGL1
  • Species:Human
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Cat. No. Size Price


RD172260100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Recombinant protein contains amino acids no. Lys 22 – Lys 249 of the protein acc.no. P54107 in UniProtKB. Total 238 AA. MW: 27 kDa (calculated). N-Terminal His-tag (10 extra AA)

Amino Acid Sequence

MKHHHHHHASKKKSARDQFNKLVTDLPNVQEEIVNIHNALRRRVVPPASNMLKMSWSEEAAQNARIFSKYCDMTESNPLERRLPNTFCGENMHMTSYPVSWSSVIGVWYSESTSFKHGEWTTTDDDITTDHYTQIVWATSYLIGCAIASCRQQGSPRYLYVCHYCHEGNDPETKNEPYKTGVPCEACPSNCEDKLCTNPCIYYDEYFDCDIQVHYLGCNHSTTILFCKATCLCDTEIK

Source

E. coli

Purity

>85%

SDS-PAGE Gel

12% SDS-PAGE separation of Human CRISP-1
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 5μg/lane
3. non-reduced and non-boiled sample,5μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Summary

Research topic

Reproduction

References to Summary

References to Cysteine-Rich Secretory Protein 1

  • Batruch I, Lecker I, Kagedan D, Smith CR, Mullen BJ, Grober E, Lo KC, Diamandis EP, Jarvi KA. Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J Proteome Res. 2011 Mar 4;10 (3):941-53
  • Cohen DJ, Da Ros VG, Busso D, Ellerman DA, Maldera JA, Goldweic N, Cuasnicu PS. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian J Androl. 2007 Jul;9 (4):528-32
  • Gibbs GM, Roelants K, O'Bryan MK. The CAP superfamily: cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins--roles in reproduction, cancer, and immune defense. Endocr Rev. 2008 Dec;29 (7):865-97
  • Koppers AJ, Reddy T, O'Bryan MK. The role of cysteine-rich secretory proteins in male fertility. Asian J Androl. 2011 Jan;13 (1):111-7
  • Kratzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD. The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3. Eur J Biochem. 1996 Mar 15;236 (3):827-36
  • Lavers AE, Swanlund DJ, Hunter BA, Tran ML, Pryor JL, Roberts KP. Acute effect of vasectomy on the function of the rat epididymal epithelium and vas deferens. J Androl. 2006 Nov-Dec;27 (6):826-36
  • Legare C, Boudreau L, Thimon V, Thabet M, Sullivan R. Vasectomy affects cysteine-rich secretory protein expression along the human epididymis and its association with ejaculated spermatozoa following vasectomy surgical reversal. J Androl. 2010 Nov-Dec;31 (6):573-83
  • Legare C, Cloutier F, Makosso-Kallyth S, Laflamme N, Jarvi K, Tremblay RR, Sullivan R. Cysteine-rich secretory protein 1 in seminal plasma: potential biomarker for the distinction between obstructive and nonobstructive azoospermia. Fertil Steril. 2013 Nov;100 (5):1253-60
  • Luo J, Yang J, Cheng Y, Li W, Yin TL, Xu WM, Zou YJ. Immunogenicity study of plasmid DNA encoding mouse cysteine-rich secretory protein-1 (mCRISP1) as a contraceptive vaccine. Am J Reprod Immunol. 2012 Jul;68 (1):47-55
  • Maldera JA, Vasen G, Ernesto JI, Weigel-Munoz M, Cohen DJ, Cuasnicu PS. Evidence for the involvement of zinc in the association of CRISP1 with rat sperm during epididymal maturation. Biol Reprod. 2011 Sep;85 (3):503-10
  • Roberts KP, Ensrud KM, Hamilton DW. A comparative analysis of expression and processing of the rat epididymal fluid and sperm-bound forms of proteins D and E. Biol Reprod. 2002 Aug;67 (2):525-33
  • Roberts KP, Ensrud KM, Wooters JL, Nolan MA, Johnston DS, Hamilton DW. Epididymal secreted protein Crisp-1 and sperm function. Mol Cell Endocrinol. 2006 May 16;250 (1-2):122-7
  • Roberts KP, Johnston DS, Nolan MA, Wooters JL, Waxmonsky NC, Piehl LB, Ensrud-Bowlin KM, Hamilton DW. Structure and function of epididymal protein cysteine-rich secretory protein-1. Asian J Androl. 2007 Jul;9 (4):508-14
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