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Cysteine-Rich Secretory Protein 1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:CRISP-1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog, AEGL1
  • Species:Human
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Cat. No. Size Price


RD191260200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Seminal plasma

Sample Requirements

5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.156–5 ng/mL

Limit of Detection

0.028 ng/mL

Intra-assay (Within-Run)

n = 8; CV = 4.1%

Inter-assay (Run-to-Run)

n = 7; CV = 5.1%

Spiking Recovery

101,60%

Dilutation Linearity

101,20%

Summary

Research topic

Reproduction

Summary

Cysteine-Rich Secretory Protein 1 (CRISP-1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog, AEGL1), 28.5 kDa epididymal protein, is a member of the cysteine-rich secretory proteins (CRISPs) family. This family of proteins is defined by the presence of 16 conserved cysteines that fold into two domains: a CAP domain, which has been implicated in cell-cell adhesion, and a CRISP domain, which has been shown to regulate several classes of ion channels across multiple species.
CRISPs show a strong expression bias to the mammalian male reproductive tract and the venom of poisonous reptiles. Within mammals, CRISPs are also expressed in lower levels within non-reproductive tissues including secretory glands, skeletal muscle, the spleen and the thymus. In the male reproductive tract CRISPs have been implicated in many aspects of male germ cell biology spanning haploid germ cell development, epididymal maturation, capacitation, motility and the actual processes of fertilization.
Human CRISP-1 is produced throughout the epididymis, whereupon it is incorporated into epididymosomes and presumably transferred onto the sperm surface. CRISP-1 was also detected as a soluble form in the seminal plasma.
The molecular mechanism of action of the mammalian CRISP proteins remains uknown, but certain non-mammalian CRISP proteins can block ion channels. CRISP-1 gene knockout mouse sperm showed reduced ability to fertilize ZP-intact and ZP-free eggs, suggesting the importance of CRISP-1 in sperm-egg fusion process. An additional role for CRISP-1 is to inhibit the uptake of ions, such as Ca2+, required for capacitation.
In the rat, CRISP-1 comprises two forms referred to as Proteins D and E. One difference between the D and E forms is an additional single O-linked N-acetyl galactosamine on an amino residue in protein E that is not present on D form. The studies demonstrate that the more abundant D form interacts with spermatozoa transiently, consistent with a capacitation-suppressing function, and the E form binds tightly and could act in the female reproductive tract.
Findings indicate vasectomy has an effect on CRISP-1 expression, because after vasectomy, the flux and composition of the epididymal fluid are modified. CRISP-1 was detected in the seminal plasma of normal and vasovasostomized men, but not in vasectomized individuals. The soluble concentration of CRISP-1 was significantly higher in the seminal plasma of vasovasostomized men when compared with normal men.
Moreover, another study has detected different levels of CRISP-1 in two types of azoospermia.
The seminal plasma samples from normospermic and nonobstructive azoospermic donors was CRISP-1 positive, whereas CRISP-1 was absent or present at low levels in samples from patients with obstructive azoospermia.

Areas of investigation: Reproduction

References to Summary

References to Cysteine-Rich Secretory Protein 1

  • Batruch I, Lecker I, Kagedan D, Smith CR, Mullen BJ, Grober E, Lo KC, Diamandis EP, Jarvi KA. Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J Proteome Res. 2011 Mar 4;10 (3):941-53
  • Cohen DJ, Da Ros VG, Busso D, Ellerman DA, Maldera JA, Goldweic N, Cuasnicu PS. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian J Androl. 2007 Jul;9 (4):528-32
  • Gibbs GM, Roelants K, O'Bryan MK. The CAP superfamily: cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins--roles in reproduction, cancer, and immune defense. Endocr Rev. 2008 Dec;29 (7):865-97
  • Koppers AJ, Reddy T, O'Bryan MK. The role of cysteine-rich secretory proteins in male fertility. Asian J Androl. 2011 Jan;13 (1):111-7
  • Kratzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD. The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3. Eur J Biochem. 1996 Mar 15;236 (3):827-36
  • Lavers AE, Swanlund DJ, Hunter BA, Tran ML, Pryor JL, Roberts KP. Acute effect of vasectomy on the function of the rat epididymal epithelium and vas deferens. J Androl. 2006 Nov-Dec;27 (6):826-36
  • Legare C, Boudreau L, Thimon V, Thabet M, Sullivan R. Vasectomy affects cysteine-rich secretory protein expression along the human epididymis and its association with ejaculated spermatozoa following vasectomy surgical reversal. J Androl. 2010 Nov-Dec;31 (6):573-83
  • Legare C, Cloutier F, Makosso-Kallyth S, Laflamme N, Jarvi K, Tremblay RR, Sullivan R. Cysteine-rich secretory protein 1 in seminal plasma: potential biomarker for the distinction between obstructive and nonobstructive azoospermia. Fertil Steril. 2013 Nov;100 (5):1253-60
  • Luo J, Yang J, Cheng Y, Li W, Yin TL, Xu WM, Zou YJ. Immunogenicity study of plasmid DNA encoding mouse cysteine-rich secretory protein-1 (mCRISP1) as a contraceptive vaccine. Am J Reprod Immunol. 2012 Jul;68 (1):47-55
  • Maldera JA, Vasen G, Ernesto JI, Weigel-Munoz M, Cohen DJ, Cuasnicu PS. Evidence for the involvement of zinc in the association of CRISP1 with rat sperm during epididymal maturation. Biol Reprod. 2011 Sep;85 (3):503-10
  • Roberts KP, Ensrud KM, Hamilton DW. A comparative analysis of expression and processing of the rat epididymal fluid and sperm-bound forms of proteins D and E. Biol Reprod. 2002 Aug;67 (2):525-33
  • Roberts KP, Ensrud KM, Wooters JL, Nolan MA, Johnston DS, Hamilton DW. Epididymal secreted protein Crisp-1 and sperm function. Mol Cell Endocrinol. 2006 May 16;250 (1-2):122-7
  • Roberts KP, Johnston DS, Nolan MA, Wooters JL, Waxmonsky NC, Piehl LB, Ensrud-Bowlin KM, Hamilton DW. Structure and function of epididymal protein cysteine-rich secretory protein-1. Asian J Androl. 2007 Jul;9 (4):508-14
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