Recombinant protein contains amino acids no. 21–245 of the protein acc.no. P54108 in UniProtKB. Total 235 AA. MW: 26.74 kDa (calculated). N-Terminal His-tag (10 extra AA)
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 85%
14 % SDS-PAGE separation of Human Cysteine-rich secretory protein 3:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5 μg/lane
3. non-reduced and non-boiled sample, 2.5 μg/lane
< 1.0 EU/ug
0.3 – 0.5 mg/ml in 50 mM acetate buffer, pH 4, 5mM DTT 20%glycerol. – filtered (0.4 μm), frozen
Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.
This product is intended for research use only.