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Manufactured by BioVendor

Dehydroepiandrosterone Sulfate ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:DHEAS
  • Species:Human
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Cat. No. Size Price

RCD008R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen



Sample Requirements

50 μl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0 .005–10 μg/ml

Limit of Detection

0.005 μg/ml

Intra-assay (Within-Run)

CV = 9.3%

Inter-assay (Run-to-Run)

CV = 9.2%

Spiking Recovery


Dilution Linearity



Research topic

Steroid hormones


Dehydroepiandros­terone sulfate (DHEAS) is produced by the adrenals and gonads. As a result, the determination of the level of DHEA-S in serum is important in the evaluation of the functional state of these glands. DHEAS is a precursor of testosterone and estrone. Besides the adrenals in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle. An abnormal testosterone level in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with Elisa of DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.

Product References (3)


  • Mostaghel EA, Cho E, Zhang A, Alyamani M, Kaipainen A, Green S, Marck BT, Sharifi N, Wright JL, Gulati R, True LD, Loda M, Matsumoto AM, Tamae D, Penning TN, Balk SP, Kantoff PW, Nelson PS, Taplin ME, Montgomery RB. Association of Tissue Abiraterone Levels and SLCO Genotype with Intraprostatic Steroids and Pathologic Response in Men with High-Risk Localized Prostate Cancer. Clin Cancer Res. 2017 Aug 15;23(16):4592-4601. doi: 10.1158/1078-0432.CCR-16-2245. Epub 2017 Apr 7. PubMed PMID: 28389510. PubMed CentralPMCID: PMC5559332. See more on PubMed
  • Stefanska A, Ponikowska I, Sypniewska G. A-FABP concentration is more strongly associated with cardiometabolic risk factors and the occurrence of metabolic syndrome in premenopausal than in postmenopausal middle-aged women. Biomed Res Int. 2014;2014:645762. doi: 10.1155/2014/645762. Epub 2014 May 29. PubMed PMID: 24971341. PubMed CentralPMCID: PMC4058146. See more on PubMed
  • Rodríguez-Gutiérrez R, Villarreal-Pérez JZ, Morales-Martinez FA, Rodríguez-Guajardo R, González-Saldivar G, Mancillas-Adame LG, Alvarez-Villalobos NA, Lavalle-Gonzalez FJ, González-González JG. Ovarian and adrenal androgens and their link to high human chorionic gonadotropin levels: a prospective controlled study. Int J Endocrinol. 2014;2014:191247. doi: 10.1155/2014/191247. Epub 2014 Nov 23. PubMed PMID: 25505909. PubMed CentralPMCID: PMC4258315. See more on PubMed
Summary References (8)

References to Dehydroepiandrosterone Sulfate

  • Chasalow FI, Blethen SL, Bradlow HL. Dehydroepiandrosterone sulfate (DHEA-S) and DHEA-S-like compounds in fibrocystic disease of the breast. Steroids. 1988 Sep;52 (3):205-15
  • Chasalow FI, Blethen SL, Duckett D, Zeitlin S, Greenfield J. Serum levels of dehydroepiandrosterone sulfate as determined by commercial kits and reagents. Steroids. 1989 Oct;54 (4):373-83
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • de Peretti E, Forest MG. Pattern of plasma dehydroepiandrosterone sulfate levels in humans from birth to adulthood: evidence for testicular production. J Clin Endocrinol Metab. 1978 Sep;47 (3):572-7
  • Holtzclaw WD, Gordon GB. Measurement of serum levels of dehydroepiandrosterone sulfate: a comparison of radioimmunoassay and enzymatic analysis. Steroids. 1989 Oct;54 (4):355-71
  • Koritnik, D.R., et al. An Elisa for DHEAS in the ciruclation of rhesus monkeys. Steroids. 42/6:653-664, 1984;
  • Orentreich N, Brind JL, Rizer RL, Vogelman JH. Age changes and sex differences in serum dehydroepiandrosterone sulfate concentrations throughout adulthood. J Clin Endocrinol Metab. 1984 Sep;59 (3):551-5
  • Smith, M.R., et al. An Elisa for the estimation of serum dehydroepiandrosterone sulfate in normal and pathological sera. Clin. Chim. Acta. 65: 5-13, 1975
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