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Dehydroepiandrosterone Sulfate ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:DHEAS
  • Species:Human
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RCD008R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample Requirements

50 μl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0 .005–10 μg/ml

Limit of Detection

0.005 μg/ml

Intra-assay (Within-Run)

CV = 9.3%

Inter-assay (Run-to-Run)

CV = 9.2%

Spiking Recovery

99,50%

Dilutation Linearity

95,70%

Summary

Research topic

Steroid hormones

Summary

Dehydroepiandros­terone sulfate (DHEAS) is produced by the adrenals and gonads. As a result, the determination of the level of DHEA-S in serum is important in the evaluation of the functional state of these glands. DHEAS is a precursor of testosterone and estrone. Besides the adrenals in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle. An abnormal testosterone level in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with Elisa of DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.

References to Product

References

  • Stefanska A, ORCID: 0000-0002-5295-2236, Ponikowska I, Sypniewska G. A-FABP concentration is more strongly associated with cardiometabolic risk factors and the occurrence of metabolic syndrome in premenopausal than in postmenopausal middle-aged women. Biomed Res Int. 2014;2014:645762
References to Summary

References to Dehydroepiandrosterone Sulfate

  • Chasalow FI, Blethen SL, Bradlow HL. Dehydroepiandrosterone sulfate (DHEA-S) and DHEA-S-like compounds in fibrocystic disease of the breast. Steroids. 1988 Sep;52 (3):205-15
  • Chasalow FI, Blethen SL, Duckett D, Zeitlin S, Greenfield J. Serum levels of dehydroepiandrosterone sulfate as determined by commercial kits and reagents. Steroids. 1989 Oct;54 (4):373-83
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • de Peretti E, Forest MG. Pattern of plasma dehydroepiandrosterone sulfate levels in humans from birth to adulthood: evidence for testicular production. J Clin Endocrinol Metab. 1978 Sep;47 (3):572-7
  • Holtzclaw WD, Gordon GB. Measurement of serum levels of dehydroepiandrosterone sulfate: a comparison of radioimmunoassay and enzymatic analysis. Steroids. 1989 Oct;54 (4):355-71
  • Koritnik, D.R., et al. An Elisa for DHEAS in the ciruclation of rhesus monkeys. Steroids. 42/6:653-664, 1984;
  • Orentreich N, Brind JL, Rizer RL, Vogelman JH. Age changes and sex differences in serum dehydroepiandrosterone sulfate concentrations throughout adulthood. J Clin Endocrinol Metab. 1984 Sep;59 (3):551-5
  • Smith, M.R., et al. An Elisa for the estimation of serum dehydroepiandrosterone sulfate in normal and pathological sera. Clin. Chim. Acta. 65: 5-13, 1975
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