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Manufactured by BioVendor

ENPP1 Protein Human HEK293

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293
  • Other names:Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, NPP-1, Plasma-cell membrane glycoprotein, PC-1
  • Species:Human
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Cat. No. Size Price

RD172124100 0.1 mg
New RD172124025 0.025 mg
PubMed Product Details
Technical Data


Recombinant protein


Total 840 AA, UniProt P22413 (Lys98-Asp925). MW: 96.5 kDa (calculated), migrates at ~ 110 kDa on SDS PAGE; N-terminal linker (2 extra AA), C-terminal linker (4 extra AA) and C-terminal His-tag (6 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence





Purity as determined by densitometric image analysis: >95%


10% SDS-PAGE separation of Human ENPP-1:
1. M.W. marker – 97, 66, 45, 31 kDa
2. reduced and boiled sample, 2.5μg / lane
3. non-reduced and non-boiled sample, 2.5μg / lane


< 0.1 EU/μg


Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4


Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.


Western blotting, ELISA, Cell culture and/or animal studies


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.


This product is intended for research use only.


Research topic

Bone and cartilage metabolism, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary References (3)

References to ENPP1

  • Cimpean A, Stefan C, Gijsbers R, Stalmans W, Bollen M. Substrate-specifying determinants of the nucleotide pyrophosphatases/phosphodiesterases NPP1 and NPP2 [published correction appears in Biochem J. 2017 Dec 14;474(24):4273-4274]. Biochem J. 2004;381(Pt 1):71-77. doi:10.1042/BJ20040465
  • Goldfine ID et al. (2010): Endocrine Rev. 29: 62-75.
  • Hessle L, Johnson KA, Anderson HC, Narisawa S, Sali A, Goding JW, Terkeltaub R, Millan JL. Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization. Proc Natl Acad Sci U S A. 2002 Jul 9;99 (14):9445-9
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