Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Urine
Sample Requirements
Serum, Plasma, Breast milk: 25 µl/well
Urine: 50 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1–40 ng/ml
Limit of Detection
0.066 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 5.8 %
Inter-assay (Run-to-Run)
n = 6; CV = 6.1 %
Spiking Recovery
96.1 %
Dilutation Linearity
97.9 %
Features
- It is intended for research use only
- The total assay time is less than 3 hours
- The kit measures total EFABP in serum, plasma (EDTA, heparin), urine, and breast milk
- Assay format is 96 wells
- Standard is a recombinant protein
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Cardiovascular disease, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Oncology
Summary
Human fatty acid binding protein EFABP is a 15 kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds ( bile acids or retinoids). in an internal cavity. The fatty acid binding proteins aP2 (fatty acid binding protein [FABP]-4) and mal1 (EFABP) are closely related and both are expressed in adipocytes. Absence of EFABP/mal1 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Adipocytes isolated from mal1-deficient mice also exhibited enhanced insulin-stimulated glucose transport capacity. In contrast, mice expressing high levels of mal1 in adipose tissue display reduced systemic insulin sensitivity .
