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Ferritin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Human
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Cat. No. Size Price


RCD012R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

20 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

10–1500 ng/ml

Limit of Detection

0.44 ng/ml

Intra-assay (Within-Run)

CV = 7.3%

Inter-assay (Run-to-Run)

CV = 4.5%

Spiking Recovery

94,40%

Dilutation Linearity

101,30%

Summary

Research topic

Iron metabolism, Oncology

Summary

Ferritin, a 450 kDa protein, is an iron storage protein containing up to 4,500 iron atoms in a molecule. It is found in many tissues but has its highest concentration in the spleen, liver, red marrow, small intestinal mucosa and muscle. The serum level of ferritin is an indirect measure of the amount of iron in the blood making it an important marker in anemia and iron metabolism research. Liver disease or malignant tumors can cause an abnormal elevation of serum ferritin levels.

Product References (2)

References

  • Ścibior A, Adamczyk A, Gołębiowska D, Niedźwiecka I. Effect of 12-weekvanadate and magnesium co-administration on chosen haematological parameters aswell as on some indices of iron and copper metabolism and biomarkers of oxidativestress in rats. Environ Toxicol Pharmacol. 2012 Sep;34(2):235-252. doi:10.1016/j.etap.2012.04.006. Epub 2012 Apr 16. PubMed PMID: 22561110. See more on PubMed
  • Kato S, Saito Y, Harada Y, Kobayashi M. Locations of peptic ulcers inchildren. Gastroenterol Jpn. 1989 Oct;24(5):476-80. PubMed PMID: 2806828. See more on PubMed
Summary References (8)

References to Ferritin

  • Bolton AE, Lee-Own V, McLean RK, Challand GS. Three different radioiodination methods for human spleen ferritin compared. Clin Chem. 1979 Oct;25 (10):1826-30
  • Drysdale JW. Ferritin as a Tumor Marker. J. Clin. Immunoassay. 6/3:234-240., 1983¨;
  • Goldie DJ, Thomas MJ. Measurement of serum ferritin by radioimmunoassay. Ann Clin Biochem. 1978 Mar;15 (2):102-8
  • Gonyea LM, Lamb CM, Sundberg RD, Deinard AS. Comparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay. Clin Chem. 1976 Apr;22 (4):513-8
  • Linpisarn S, Kricka LJ, Kennedy JH, Whitehead TP. Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates. Ann Clin Biochem. 1981 Jan;18 (Pt 1):48-53
  • Luxton AW, Walker WH, Gauldie J, Ali AM, Pelletier C. A radioimmunoassay for serum ferritin. Clin Chem. 1977;23 (4):683-9
  • Ng RH, Brown BA, Valdes R Jr. Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. Clin Chem. 1983 Jun;29 (6):1109-13
  • Vernon AP, et al. Ferritin: A Reliable Assay for Detection of Iron Deficiency. The Ligand Quarterly. 3/4:15-16, 1980
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