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Ferritin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Human
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Cat. No. Size Price

RCD012R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody



Sample Requirements

20 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

10–1500 ng/ml

Limit of Detection

0.44 ng/ml

Intra-assay (Within-Run)

CV = 7.3%

Inter-assay (Run-to-Run)

CV = 4.5%

Spiking Recovery


Dilutation Linearity



Research topic

Iron metabolism, Oncology


Ferritin, a 450 kDa protein, is an iron storage protein containing up to 4,500 iron atoms in a molecule. It is found in many tissues but has its highest concentration in the spleen, liver, red marrow, small intestinal mucosa and muscle. The serum level of ferritin is an indirect measure of the amount of iron in the blood making it an important marker in anemia and iron metabolism research. Liver disease or malignant tumors can cause an abnormal elevation of serum ferritin levels.

References to Product


  • Scibior A, Adamczyk A, Golebiowska D, Niedzwiecka I. Effect of 12-week vanadate and magnesium co-administration on chosen haematological parameters as well as on some indices of iron and copper metabolism and biomarkers of oxidative stress in rats. Environ Toxicol Pharmacol. 2012 Sep;34 (2):235-52
  • Stefanova KI, Delcheva GT, Maneva AI, Batalov AZ, Geneva-Popova MG, Karalilova RV, Simitchiev KK. Pathobiochemical Mechanisms Relating Iron Homeostasis to Parameters of Inflammatory Activity and Autoimmune Disorders in Rheumatoid Arthritis. Folia Med (Plovdiv). 2016 Dec 1;58(4):257-263. doi: 10.1515/folmed-2016-0040
References to Summary

References to Ferritin

  • Bolton AE, Lee-Own V, McLean RK, Challand GS. Three different radioiodination methods for human spleen ferritin compared. Clin Chem. 1979 Oct;25 (10):1826-30
  • Drysdale JW. Ferritin as a Tumor Marker. J. Clin. Immunoassay. 6/3:234-240., 1983¨;
  • Goldie DJ, Thomas MJ. Measurement of serum ferritin by radioimmunoassay. Ann Clin Biochem. 1978 Mar;15 (2):102-8
  • Gonyea LM, Lamb CM, Sundberg RD, Deinard AS. Comparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay. Clin Chem. 1976 Apr;22 (4):513-8
  • Linpisarn S, Kricka LJ, Kennedy JH, Whitehead TP. Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates. Ann Clin Biochem. 1981 Jan;18 (Pt 1):48-53
  • Luxton AW, Walker WH, Gauldie J, Ali AM, Pelletier C. A radioimmunoassay for serum ferritin. Clin Chem. 1977;23 (4):683-9
  • Ng RH, Brown BA, Valdes R Jr. Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. Clin Chem. 1983 Jun;29 (6):1109-13
  • Vernon AP, et al. Ferritin: A Reliable Assay for Detection of Iron Deficiency. The Ligand Quarterly. 3/4:15-16, 1980
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