Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Fetuin-B Human HEK293

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293
  • Other names:16G2, Fetuin-like protein IRL685, Gugu, FETUB
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD172172100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

MW: 41.8 kDa (calculated), migrates at ~ 55 kDa on SDS-PAGE; N-terminal linker (4 extra AA highlighted), C-terminal His-tag (6 extra AA highlighted). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

HSHVCGAMSPPQLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYNNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPPHHHHHH

Source

HEK293

Purity

Purity as determined by densitometric image analysis: >90%

SDS-PAGE Gel

12% SDS-PAGE separation of Human Fetuin-B
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 5μg / lane
3. non-reduced and non-boiled sample, 5μg / lane

Endotoxin

<0.1 EU/µg

Formulation

Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA, Cell culture and/or animal studies

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Summary

Research topic

Bone and cartilage metabolism, Energy metabolism and body weight regulation, Immune Response, Infection and Inflammation, Oncology, Others

Summary

Fetuin B is a member of cystatin superfamily proteins, comprises two cystatin domains. It was found in secreted form in serum, it is expressed in liver, tongue and placenta. It regulates osteogenesis by inhibition effect on mineralization. It plays a role in oocyte maturation.

References to Summary

References to Fetuin-B

  • Arnaud P, Miribel L, Emerson DL. Alpha 2-HS glycoprotein. Methods Enzymol. 1988;163:431-41
  • Choi JW, Aseer KR, Chaudhari HN, Mukherjee R, Choi M, Yun JW. Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats. Proteomics. 2013 Aug;13 (16):2482-94
  • Choi JW, Liu H, Mukherjee R, Yun JW. Downregulation of fetuin-B and zinc-alpha2-glycoprotein is linked to impaired fatty acid metabolism in liver cells. Cell Physiol Biochem. 2012;30 (2):295-306
  • Denecke B, Graber S, Schafer C, Heiss A, Woltje M, Jahnen-Dechent W. Tissue distribution and activity testing suggest a similar but not identical function of fetuin-B and fetuin-A. Biochem J. 2003 Nov 15;376 (Pt 1):135-45
  • Dietzel E, Wessling J, Floehr J, Schafer C, Ensslen S, Denecke B, Rosing B, Neulen J, Veitinger T, Spehr M, Tropartz T, Tolba R, Renne T, Egert A, Schorle H, Gottenbusch Y, Hildebrand A, Yiallouros I, Stocker W, Weiskirchen R, Jahnen-Dechent W. Fetuin-B, a liver-derived plasma protein is essential for fertilization. Dev Cell. 2013 Apr 15;25 (1):106-12
  • Gharbi M, Sanchez C, Mazzucchelli G, De Pauw E, Henrotin Y. Identification of differential pattern of protein expression in canine osteoarthritis serum after anterior cruciate ligament transection: a proteomic analysis. Vet J. 2013 Sep;197 (3):848-53
  • Hsu SJ, Nagase H, Balmain A. Identification of Fetuin-B as a member of a cystatin-like gene family on mouse chromosome 16 with tumor suppressor activity. Genome. 2004 Oct;47 (5):931-46
  • Murakami T, Walczak R, Caron S, Duhem C, Vidal V, Darteil R, Staels B. The farnesoid X receptor induces fetuin-B gene expression in human hepatocytes. Biochem J. 2007 Nov 1;407 (3):461-9
  • Olivier E, Soury E, Risler JL, Smih F, Schneider K, Lochner K, Jouzeau JY, Fey GH, Salier JP. A novel set of hepatic mRNAs preferentially expressed during an acute inflammation in rat represents mostly intracellular proteins. Genomics. 1999 May 1;57 (3):352-64
  • Olivier E, Soury E, Ruminy P, Husson A, Parmentier F, Daveau M, Salier JP. Fetuin-B, a second member of the fetuin family in mammals. Biochem J. 2000 Sep 1;350 Pt 2:589-97
  • Palviainen M, Raekallio M, Vainionpaa M, Kosonen S, Vainio O. Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis. Toxicon. 2012 Dec 1;60 (7):1228-34
  • Pan TL, Wang PW, Al-Suwayeh SA, Huang YJ, Fang JY. Toxicological effects of cationic nanobubbles on the liver and kidneys: biomarkers for predicting the risk. Food Chem Toxicol. 2012 Nov;50 (11):3892-901
  • Rosenling T, Stoop MP, Attali A, van Aken H, Suidgeest E, Christin C, Stingl C, Suits F, Horvatovich P, Hintzen RQ, Tuinstra T, Bischoff R, Luider TM. Profiling and identification of cerebrospinal fluid proteins in a rat EAE model of multiple sclerosis. J Proteome Res. 2012 Apr 6;11 (4):2048-60
  • Schroeder AC, Schultz RM, Kopf GS, Taylor FR, Becker RB, Eppig JJ. Fetuin inhibits zona pellucida hardening and conversion of ZP2 to ZP2f during spontaneous mouse oocyte maturation in vitro in the absence of serum. Biol Reprod. 1990 Nov;43 (5):891-7
  • Woltje M, Tschoke B, von Bulow V, Westenfeld R, Denecke B, Graber S, Jahnen-Dechent W. CCAAT enhancer binding protein beta and hepatocyte nuclear factor 3beta are necessary and sufficient to mediate dexamethasone-induced up-regulation of alpha2HS-glycoprotein/fetuin-A gene expression. J Mol Endocrinol. 2006 Apr;36 (2):261-77
Related Products Documents