Type
Isolation kit- small RNA
Applications
Frozen or paraffin tissue sections
Sample Requirements
5-10um section FFPE slide
Storage/Expiration
Store buffer, solvent and consumables at 15-25°C, MR23 Column at 4℃, Proteinase K at -20 ℃, all components are stable until the expiration date (see label on the box).
Features
It is intended for research use only.
The total assay time is less than 45 minutes.
The kit isolates short RNA molecules from FFPE tissue samples.
One kit is complete for 50 samples isolation.
Research topic
Apoptosis, Autoimmunity, Bone and cartilage metabolism, Cell apoptosis, Cell.cycle and Cell migration, Immune Response, Infection and Inflammation, Oncology, Oxidative stress, Serology, Transplantation
Summary
The FFPE miRNA Kit is designed to purify 19-24 nucleotides miRNA, small RNA and less than 1000 nucleotides RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissue specimens. Based on optimized reagent buffer and silica membrane column, this kit is able to get high quality and purity of miRNA, which can be used in wide range of downstream application such as qPCR, Microarray and NGS. It provides a convenient and eco-friendly protocol without using phenol or chloroform for RNA purification.