Type
Recombinant protein
Description
Total 203 AA. MW: 23.78 kDa (calculated). UniProtKB acc.no. O35622. N-Terminal His-tag, 10 extra AA (highlighted).
Amino Acid Sequence
MKHHHHHHASRPLAQQSQSVSDEDPLFLYGWGKITRLQYLYSAGPYVSNCFLRIRSDGSVDCEEDQNERNLLEFRAVALKTIAIKDVSSVRYLCMSADGKIYGLIRYSEEDCTFREEMDCLGYNQYRSMKHHLHIIFIQAKPREQLQDQKPSNFIPVFHRSFFETGDQLRSKMFSLPLESDSMDPFRMVEDVDHLVKSPSFQK
Source
E. coli
Purity
Purity as determined by densitometric image analysis: >90%
SDS-PAGE Gel
12% SDS-PAGE separation of Mouse FGF15
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 5μg/lane
3. non-reduced and non-boiled sample, 5μg/lane
Endotoxin
< 0.1 EU/μg
Formulation
Filtered (0.4 μm) and frozen from 0.2 – 0.5 mg/mL solution in 20mM TRIS, 50mM NaCl, pH = 8.5
Reconstitution
Defrost at ambient temperature
Applications
Western blotting
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles. The thawed protein can be stored at 4 °C for three days.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
Note
This product is intended for research use only.