Total 296 AA. MW: 33.8 kDa (calculated). UniProtKB acc. No. Q12841 (Glu21–Ile308). C-terminal His-tag (8 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95%
12 % SDS-PAGE separation of Human FSTL1 (HEK):
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane
< 1.0 EU/μg
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in phosphate buffered saline, 5% w/v trehalose, pH 7.4
Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA
At temperature 2 – 8 °C. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
This product is intended for research use only.