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Manufactured by BioVendor

Free beta-Chorionic Gonadotropin (beta-hCG) Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:hCG
  • Species:Human
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Cat. No. Size Price

RIS0011R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody



Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

10–200 ng/ml

Limit of Detection

0.2 ng/ml


Research topic



Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. The hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Structurally intact hCG molecules consist of two non-covalently linked polypeptide subunits, the alpha and beta chain subunits. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. The measurement of free β-hCG in the first trimester of pregnancy has been reported as a useful marker in antenatal screening for Down Syndrome and other fetal aneuploidies. Increased free β-hCG values in combination with maternal age, the measurement of PAPP-A and the ultrasonic determination of nuchal translucency (NT) in pregnancy weeks 11 to 14 may detect up to 90 % of pregnancies with Down syndrom (reference 15).

The Biovendor free β-hCG ELISA may be used for the risk assessment of Down´s syndrom (trisomy 21) in the first trimester of pregnancy. For the risk assessment of trisomy 21 and other fetal aneuploidies free beta hCG should always be measured in combination with other analytes (for example PAPP-A and NT, see above) and a special
software for the risk assessment of trisomy 21. According to the IVD Directive (98/79/EC) both software and kits for the additional analytes must be suitable for trisomy 21 screening and CE-certified by a notified body, indicated by the identification number of the notified body on the CE-mark on software and kits.

Product References (12)


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  • Nobles CJ, Mendola P, Mumford SL, Naimi AI, Yeung EH, Kim K, Park H, Wilcox B,Silver RM, Perkins NJ, Sjaarda L, Schisterman EF. Preconception Blood PressureLevels and Reproductive Outcomes in a Prospective Cohort of Women AttemptingPregnancy. Hypertension. 2018 May;71(5):904-910. doi:10.1161/HYPERTENSIONAHA.117.10705. Epub 2018 Apr 2. PubMed PMID: 29610265; PubMedCentral PMCID: PMC5897130. See more on PubMed
  • Plowden TC, Schisterman EF, Sjaarda LA, Zarek SM, Perkins NJ, Silver R, Galai N, DeCherney AH, Mumford SL. Subclinical Hypothyroidism and Thyroid Autoimmunity Are Not Associated With Fecundity, Pregnancy Loss, or Live Birth. J ClinEndocrinol Metab. 2016 Jun;101(6):2358-65. doi: 10.1210/jc.2016-1049. Epub 2016Mar 29. PubMed PMID: 27023447; PubMed Central PMCID: PMC4891792. See more on PubMed
  • Pugh SJ, Schisterman EF, Browne RW, Lynch AM, Mumford SL, Perkins NJ, SilverR, Sjaarda L, Stanford JB, Wactawski-Wende J, Wilcox B, Grantz KL. Preconception maternal lipoprotein levels in relation to fecundability. Hum Reprod. 2017 May1;32(5):1055-1063. doi: 10.1093/humrep/dex052. PubMed PMID: 28333301; PubMedCentral PMCID: PMC6075456. See more on PubMed
  • Radin RG, Mumford SL, Sjaarda LA, Silver RM, Wactawski-Wende J, Lynch AM,Perkins NJ, Lesher LL, Wilcox BD, Hinkle SN, Plowden TC, Kim K, Schisterman EF.Recent attempted and actual weight change in relation to pregnancy loss: aprospective cohort study. BJOG. 2018 May;125(6):676-684. doi:10.1111/1471-0528.14859. Epub 2017 Oct 25. PubMed PMID: 29067752; PubMed Central PMCID: PMC5918461. See more on PubMed
  • Radin RG, Sjaarda LA, Perkins NJ, Silver RM, Chen Z, Lesher LL, Galai N,Wactawski-Wende J, Mumford SL, Schisterman EF. Low-Dose Aspirin and SporadicAnovulation in the EAGeR Randomized Trial. J Clin Endocrinol Metab. 2017 Jan1;102(1):86-92. doi: 10.1210/jc.2016-2095. PubMed PMID: 27754808; PubMed Central PMCID: PMC5413097. See more on PubMed
  • Radin RG, Sjaarda LA, Silver RM, Nobles CJ, Mumford SL, Perkins NJ, Wilcox BD,Pollack AZ, Schliep KC, Plowden TC, Schisterman EF. C-Reactive protein inrelation to fecundability and anovulation among eumenorrheic women. FertilSteril. 2018 Feb;109(2):232-239.e1. doi: 10.1016/j.fertnstert.2017.10.025. Epub2018 Jan 6. PubMed PMID: 29317123; PubMed Central PMCID: PMC5815923. See more on PubMed
  • Russo LM, Whitcomb BW, Mumford SL, Hawkins M, Radin RG, Schliep KC, Silver RM,Perkins NJ, Kim K, Omosigho UR, Kuhr DL, Holland TL, Sjaarda LA, Schisterman EF. A prospective study of physical activity and fecundability in women with ahistory of pregnancy loss. Hum Reprod. 2018 Jul 1;33(7):1291-1298. doi:10.1093/humrep/dey086. PubMed PMID: 29648647; PubMed Central PMCID: PMC6012250. See more on PubMed
  • Schisterman EF, Mumford SL, Schliep KC, Sjaarda LA, Stanford JB, Lesher LL,Wactawski-Wende J, Lynch AM, Townsend JM, Perkins NJ, Zarek SM, Tsai MY, Chen Z, Faraggi D, Galai N, Silver RM. Preconception low dose aspirin and time topregnancy: findings from the effects of aspirin in gestation and reproductionrandomized trial. J Clin Endocrinol Metab. 2015 May;100(5):1785-91. doi:10.1210/jc.2014-4179. Epub 2015 Feb 24. PubMed PMID: 25710565; PubMed CentralPMCID: PMC4422888. See more on PubMed
  • Zarek SM, Mitchell EM, Sjaarda LA, Mumford SL, Silver RM, Stanford JB, GalaiN, Schliep KC, Radin RG, Plowden TC, DeCherney AH, Schisterman EF. Antimüllerian hormone and pregnancy loss from the Effects of Aspirin in Gestation andReproduction trial. Fertil Steril. 2016 Apr;105(4):946-952.e2. doi:10.1016/j.fertnstert.2015.12.003. Epub 2015 Dec 17. PubMed PMID: 26707905; PubMedCentral PMCID: PMC4821799. See more on PubMed
  • Zarek SM, Mitchell EM, Sjaarda LA, Mumford SL, Silver RM, Stanford JB, GalaiN, White MV, Schliep KC, DeCherney AH, Schisterman EF. Is Anti-Müllerian Hormone Associated With Fecundability? Findings From the EAGeR Trial. J Clin EndocrinolMetab. 2015 Nov;100(11):4215-21. doi: 10.1210/jc.2015-2474. Epub 2015 Sep 25.PubMed PMID: 26406293; PubMed Central PMCID: PMC4702454. See more on PubMed
Summary References (22)

References to Chorionic Gonadotropin

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  • Milwidsky A, Adoni A, Miodovnik M, Segal S, Palti Z. Human chorionic gonadotropin (beta-subunit) in the early diagnosis of ectopic pregnancy. Obstet Gynecol. 1978 Jun;51 (6):725-6
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