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FSH Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Follicle Stimulating Hormone
  • Species:Human
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RCD013R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

25 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

5–100 IU/l

Limit of Detection

1 IU/l

Spiking Recovery

98,20%

Dilutation Linearity

106,40%

Summary

Research topic

Reproduction

Summary

Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) are intimately involved in the control of the growth and reproductive activities of the gonadal tissues, which synthesize and secrete male and female sex hormones through a negative feedback relationship. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar structurally, therefore the biological and immunological properties of each are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the granulosa cells; follicular steroidogenesis is promoted and LH production is stimulated. The LH produced then binds to the theca cells and stimulates steroidogenesis. Increased intraovarian estradiol production occurs as follicular maturation advances, thereupon stimulating increased FSH receptor activity and FSH follicular binding. FSH, LH, and estradiol are therefore intimately related in supporting ovarian recruitment and maturation in women. FSH levels are elevated after menopause, castration, and in premature ovarian failure. The levels of FSH may be normalized through the administration of estrogens, which demonstrate a negative feedback mechanism. Abnormal relationships between FSH and LH, between FSH and estrogen have been linked to anorexia nervosa and polycystic ovarian disease. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/mL. The growth of the seminiferous tubules and maintenance of spermatogenesis in men are regulated by FSH. However, androgens, unlike estrogens, do not lower FSH levels, therefore demonstrating a feedback relationship only with serum LH. For reasons not fully understood, azospermic and oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated, as determined by radioimmunoassay. It has been postulated that the apparent LH increase may be caused by crossreactivity with hCG-like substances secreted by tumors of the testes. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis.

References to Summary

References to FSH

  • Butt WR, Ryle M, Shirley A. Kinetic studies with 125I-labelled follicle-stimulating hormone. J Endocrinol. 1973 Aug;58 (2):275-87
  • Cohen KL. Metabolic, endocrine, and drug-induced interference with pituitary function tests: a review. Metabolism. 1977 Oct;26 (10):1165-77
  • Jeffcoate SL. The control of testicular function in the adult. Clin Endocrinol Metab. 1975 Nov;4 (3):521-43
  • Jockenhovel F, Khan SA, Nieschlag E. Circulating antibodies to monoclonal immunoglobulins used in a follitropin assay may cause incorrect fertility diagnosis. J Clin Chem Clin Biochem. 1989 Oct;27 (10):825-8
  • Kjeld JM, Harsoulis P, Kuku SF, Marshall JC, Kaufman B, Fraser TR. Infusions of hFSH and hLH in normal men. I. Kinetics of human follicle stimulating hormone. Acta Endocrinol (Copenh). 1976 Feb;81 (2):225-33
  • Knobil E. The neuroendocrine control of the menstrual cycle. Recent Prog Horm Res. 1980;36:53-88
  • Leonard JM, Leach RB, Couture M, Paulsen CA. Plasma and urinary follicle-stimulating hormone levels in oligospermia. J Clin Endocrinol Metab. 1972 Jan;34 (1):209-14
  • Lundy LE, Lee SG, Levy W, Woodruff JD, Wu CH, Abdalla M. The ovulatory cycle. A histologic, thermal, steroid, and gonadotropin correlation. Obstet Gynecol. 1974 Jul;44 (1):14-25
  • Marshall JC. Clinics in endocrinology and metabolism. Investigative procedures. Clin Endocrinol Metab. 1975 Nov;4 (3):545-67
  • Rebar RW, Erickson GF, Yen SS. Idiopathic premature ovarian failure: clinical and endocrine characteristics. Fertil Steril. 1982 Jan;37 (1):35-41
  • Reiter EO, Kulin HE. Suppressed follicle stimulating hormone in men with chorionic gonadotropin secreting testicular tumors. J Clin Endocrinol Metab. 1971 Dec;33 (6):957-61
  • Seth J, Hanning I, Bacon RR, Hunter WM. Progress and problems in immunoassays for serum pituitary gonadotrophins: evidence from the UK external quality assessment schemes, (EQAS) 1980-1988. Clin Chim Acta. 1989 Dec 29;186 (1):67-82
  • Shome B, Parlow AF. Human follicle stimulating hormone (hFSH): first proposal for the amino acid sequence of the alpha-subunit (hFSHa) and first demonstration of its identity with the alpha-subunit of human luteinizing hormone (hLHa). J Clin Endocrinol Metab. 1974 Jul;39 (1):199-202
  • Shome B, Parlow AF. Human follicle stimulating hormone: first proposal for the amino acid sequence of the hormone-specific, beta subunit (hFSHb). J Clin Endocrinol Metab. 1974 Jul;39 (1):203-5
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
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