Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant Human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55–66 kDa by SDS-PAGE analysis under non-reducing conditions. The calculated molecular weight of Recombinant Human GASP-1 is 59.9 kDa.
Amino Acid Sequence
Chinese Hamster Ovary Cells (CHO)
Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED 50 for this effect is 0.0025–0.0040 µg/ml in the presence of 5ng/ml of human Myostatin (GDF-8).
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.