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Manufactured by BioVendor

Gastrokine-1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:18 kDa antrum mucosa protein, AMP-18, CA11, GKN1, AMP18, UNQ489/PRO1005
  • Species:Human
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Cat. No. Size Price


RD191268200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

35 µl/well

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.5 – 32 ng/ml

Limit of Detection

0.035 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 2.3 %

Inter-assay (Run-to-Run)

n = 6; CV = 7.8 %

Spiking Recovery

101.8 %

Dilutation Linearity

108.1 %

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures GKN1 in serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Oncology

Summary

Gastrokine 1, previously known as AMP-18, CA11, FOVEOLIN and TFIZ, was formally named “GKN1” by the HUGO gene Nomenclature Committee for its gastric-specific expression and its highly conserved presence in the gastric mucosa of many mammalian species. Gastrokine 1 is a novel protein that was firstly cloned by a Japanese group in 2000. GKN1 belongs to a family of genes encoding stomach-specific secreted proteins consisting of 3 known members: gastrokine 1 (GKN1), gastrokine 2 (GKN2) and gastrokine 3 (GKN3). GKN1 gene of about 6 kb was reported to be located at 2p13 and contains 6 exons. The GKN1 gene encodes a small protein of 185 amino acids containing a N-terminal signal peptide. It is a secreted protein with a molecular weight of approximately 18 kDa. GKN1 protein contains a BRICHOS domain, which is associated with dementia, respiratory distress and cancer. Molecular studies on the BRICHOS domain function have suggested that it has a range of possible roles, including intracellular trafficking, propeptide processing, chaperone function and secretion. However, the biological activities of the BRICHOS domain in GKN1 have not been elucidated. Gastrokine 1 has been detected recently in normal gastric mucosa, but not in other regions of the gastrointestinal tract. Immunoelectron microscopy indicated that the GKN1 protein is localized within the granules just under the apical plasma membrane. These expression patterns of GKN1 indicate that it may play a distinctive role in the stomach. Functionally, GKN1 promotes the maturation of gastric mucosa, and maintains the integrity of gastric mucosal epithelium through mitogenic and mutagenic abilities. GKN1 may also protect the intestinal mucosal barrier by acting on specific tight junction proteins and stabilizing perijunctional actin. If the protein is downregulated, the repair process may be impaired. Recently, a number of studies have found that deficiency of GKN1 can result in instability of the gastric epithelium. Clinically, GKN1 is downregulated in Helicobacter pylori-infected gastric epithelial cells, and this loss of GKN1 expression is detected in gastric cancer and precancerous lesion, such as intestinal metaplasia. Invasive factor such as H. pylori contribute to the downregulation of GKN1, whilst inducing ulceration and cancer. Moreover, GKN1 plays an important role in epithelial-mesenchymal transition and migration of gastric cancer cells by regulating reactive oxygen species (ROS) and the P13K/Akt pathway. Recently, it has been suggested that GKN1 induces senescence through activating p16/Rb pathway in gastric cancer cells. Thus, GKN1 may play a key role in the progression of gastric cancer, and may be a potential biomarker for the early detection of gastric cancer.

References to Summary

References to Gastrokine-1

  • Choi WS, Seo HS, Song KY, Yoon JH, Kim O, Nam SW, Lee JY, Park WS. Gastrokine 1 expression in the human gastric mucosa is closely associated with the degree of gastritis and DNA methylation. J Gastric Cancer. 2013 Dec;13 (4):232-41
  • Guo XY, Dong L, Qin B, Jiang J, Shi AM. Decreased expression of gastrokine 1 in gastric mucosa of gastric cancer patients. World J Gastroenterol. 2014 Nov 28;20 (44):16702-6
  • Mao W, Chen J, Peng TL, Yin XF, Chen LZ, Chen MH. Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis. J Exp Clin Cancer Res. 2012;31:49
  • Mao W, Chen J, Peng TL, Yin XF, Chen LZ, Chen MH. Helicobacter pylori infection and administration of non-steroidal anti-inflammatory drugs down-regulate the expression of gastrokine-1 in gastric mucosa. Turk J Gastroenterol. 2012 Jun;23 (3):212-9
  • Moss SF, Lee JW, Sabo E, Rubin AK, Rommel J, Westley BR, May FE, Gao J, Meitner PA, Tavares R, Resnick MB. Decreased expression of gastrokine 1 and the trefoil factor interacting protein TFIZ1/GKN2 in gastric cancer: influence of tumor histology and relationship to prognosis. Clin Cancer Res. 2008 Jul 1;14 (13):4161-7
  • Nardone G, Martin G, Rocco A, Rippa E, La Monica G, Caruso F, Arcari P. Molecular expression of Gastrokine 1 in normal mucosa and in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions. Cancer Biol Ther. 2008 Dec;7 (12):1890-5
  • Rippa E, Mallardo P, Arcari P. Role of Gastrokine 1 in Gastric Cancer. J of Gastritis and Gastric Cancer – New Insights in Gastroprotection, Diagnosis and Treatments. InTech, Dr. Paola Tonino (Ed.). 2011;ISBN: 978-953-307-375-0:281-296
  • Xiao JW, Chen JH, Ren MY, Tian XB, Wang CS. Relationship between expression of gastrokine 1 and clinicopathological characteristics in gastric cancer patients. Asian Pac J Cancer Prev. 2012;13 (11):5897-901
  • Yoon JH, Cho ML, Choi YJ, Back JY, Park MK, Lee SW, Choi BJ, Ashktorab H, Smoot DT, Nam SW, Lee JY, Park WS. Gastrokine 1 regulates NF-kappaB signaling pathway and cytokine expression in gastric cancers. J Cell Biochem. 2013 Aug;114 (8):1800-9
  • Yoon JH, Choi WS, Kim O, Park WS. The role of gastrokine 1 in gastric cancer. J Gastric Cancer. 2014 Sep;14 (3):147-55
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