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Manufactured by BioVendor

GDF-15/MIC-1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Growth differentiation factor 15
  • Species:Human
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Cat. No. Size Price


RD191135200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma

Sample Requirements

30 μl/well

Storage/Expiration

Store the complete kit at 2 – 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

35–4480 pg/ml

Limit of Detection

16 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 6.8%

Inter-assay (Run-to-Run)

n = 8; CV = 4.3%

Spiking Recovery

96,30%

Dilutation Linearity

93,40%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • monkey Yes (recommended dilution 1:3)
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 3.5 hours.
  • The kit measures Human GDF-15 in serum, plasma (EDTA, citrate, heparin).
  • Assay format is 96 wells.
  • Quality Controls are human serum based. No animal sera are used.
  • Standard is recombinant protein based.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Cardiovascular disease, Cytokines and chemokines and related molecules, Oncology, Reproduction

Summary

GDF-15/MIC-1 is synthesized as a 62-kDa precursor protein, which, after cleavage by furin-like protease, is secreted as 25-kDa disulfide-linked dimmer. GDF-15/MIC-1 is weakly produced under baseline condition in most tissues such as brain, liver, kidney, pancreas, but not normally in many other organs including the heart, and is highly expressed in placenta and moderately in prostate. GDF-15 is upregulated by cardiovascular events triggering oxidative stress, including pressure overload, and atherosclerosis. Moreover, increased circulating GDF-15 concentrations have been linked to an enhanced risk of future adverse cardiovascular events in elderly woman and it was describe as a new biomarker of the risk of death in patients with non-ST-elevation acute coronary syndrome. Serum GDF-15 concentrations increase in maternal serum with advancing gestation in normal pregnancy. Low GDF-15 concentrations reportedly are associated with an increased risk of preterm labor or miscarriage.

References to Product

References

  • Arslan D, Cihan T, Kose D, Vatansev H, Cimen D, Koksal Y, Oran B, Akyurek F. Growth-differentiation factor-15 and tissue doppler imaging in detection of asymptomatic anthracycline cardiomyopathy in childhood cancer survivors. Clin Biochem. 2013 Sep;46 (13-14):1239-43
  • Arslan D, Cihan T, Kose D, Vatansev H, Cimen D, Koksal Y, Oran B, Akyurek F. Growth-differentiation factor-15 and tissue doppler imaging in detection of asymptomatic anthracycline cardiomyopathy in childhood cancer survivors. Clin Biochem. 2013 Sep;46 (13-14):1239-43
  • Dostalova I, Kavalkova P, Papezova H, Domluvilova D, Zikan V, Haluzik M. Association of macrophage inhibitory cytokine-1 with nutritional status, body composition and bone mineral density in patients with anorexia nervosa: the influence of partial realimentation. Nutr Metab (Lond). 2010;7:34
  • Dostalova I, Roubicek T, Bartlova M, Mraz M, Lacinova Z, Haluzikova D, Kavalkova P, Matoulek M, Kasalicky M, Haluzik M. Increased serum concentrations of macrophage inhibitory cytokine-1 in patients with obesity and type 2 diabetes mellitus: the influence of very low calorie diet. Eur J Endocrinol. 2009 Sep;161 (3):397-404
  • Izumiya Y, Hanatani S, Kimura Y, Takashio S, Yamamoto E, Kusaka H, Tokitsu T, Rokutanda T, Araki S, Tsujita K, Tanaka T, Yamamuro M, Kojima S, Tayama S, Kaikita K, Hokimoto S, Ogawa H. Growth differentiation factor-15 is a useful prognostic marker in patients with heart failure with preserved ejection fraction. Can J Cardiol. 2014 Mar;30 (3):338-44
  • Montoro-Garcia S, Hernandez-Romero D, Jover E, Garcia-Honrubia A, Vilchez JA, Casas T, Martinez P, Climent V, Caballero L, Valdes M, Marin F. Growth differentiation factor-15, a novel biomarker related with disease severity in patients with hypertrophic cardiomyopathy. Eur J Intern Med. 2012 Mar;23 (2):169-74
  • Stejskal D, Karpisek M, Humenanska V, Lacnak B, Svestak M. Macrophage-inhibitory cytokine-1 (mic-1) in differential diagnosis of dyspnea-A pilot study. Clin Biochem. 2009 Mar 31;
  • Yalcin MM, Altinova AE, Akturk M, Gulbahar O, Arslan E, Sendogan DO, Yetkin I, Toruner DB. GDF-15 AND HEPCIDIN LEVELS IN NON-ANEMIC PATIENTS WITH IMPAIRED GLUCOSE TOLERANCE. August 2016;
  • SAVCHUK, N. V.; VAKALIUK, I. P.. Dynamics of Heart Failure Markers in Patients after Past Myocardial Infarction with the Use of Potassium and Magnesium Salts of Gluconic Acid, Eplerenone and Rivaroxaban. Archive of Clinical Medicine, [S.l.], v. 23, n. 1, june 2017. ISSN 2414-9853. Available at: http://ojs.ifnmu.edu.ua/index.php/acm/article/view/772/720. doi:http://dx.doi.org/10.21802/acm.2017.1.10.
  • Jenab Y, Pourjafari M, Sotoudeh M, Lotfi-Tokaldany M, Etesamifard N, Shirani S, Jalali A, Nozari Y, Poorhosseini HR. Comparing the effect of cardiac biomarkers on the outcome of normotensive patients with acute pulmonary embolism. Monaldi Arch Chest Dis. 2017 May 18;87(1):767. doi: 10.4081/monaldi.2017.767.
References to Summary

References to GDF-15/MIC-1

  • Ago T, Sadoshima J. GDF15, a cardioprotective TGF-beta superfamily protein. Circ Res. 2006 Feb 17;98 (3):294-7
  • Bootcov MR, Bauskin AR, Valenzuela SM, Moore AG, Bansal M, He XY, Zhang HP, Donnellan M, Mahler S, Pryor K, Walsh BJ, Nicholson RC, Fairlie WD, Por SB, Robbins JM, Breit SN. MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily. Proc Natl Acad Sci U S A. 1997 Oct 14;94 (21):11514-9
  • Brown DA, Moore J, Johnen H, Smeets TJ, Bauskin AR, Kuffner T, Weedon H, Milliken ST, Tak PP, Smith MD, Breit SN. Serum macrophage inhibitory cytokine 1 in rheumatoid arthritis: a potential marker of erosive joint destruction. Arthritis Rheum. 2007 Mar;56 (3):753-64
  • Brown DA, Ward RL, Buckhaults P, Liu T, Romans KE, Hawkins NJ, Bauskin AR, Kinzler KW, Vogelstein B, Breit SN. MIC-1 serum level and genotype: associations with progress and prognosis of colorectal carcinoma. Clin Cancer Res. 2003 Jul;9 (7):2642-50
  • Eggers KM, Kempf T, Allhoff T, Lindahl B, Wallentin L, Wollert KC. Growth-differentiation factor-15 for early risk stratification in patients with acute chest pain. Eur Heart J. 2008 Oct;29 (19):2327-35
  • Huang CY, Beer TM, Higano CS, True LD, Vessella R, Lange PH, Garzotto M, Nelson PS. Molecular alterations in prostate carcinomas that associate with in vivo exposure to chemotherapy: identification of a cytoprotective mechanism involving growth differentiation factor 15. Clin Cancer Res. 2007 Oct 1;13 (19):5825-33
  • Keelan JA, Wang K, Chaiworapongsa T, Romero R, Mitchell MD, Sato TA, Brown DA, Fairlie WD, Breit SN. Macrophage inhibitory cytokine 1 in fetal membranes and amniotic fluid from pregnancies with and without preterm labour and premature rupture of membranes. Mol Hum Reprod. 2003 Sep;9 (9):535-40
  • Kempf T, Bjorklund E, Olofsson S, Lindahl B, Allhoff T, Peter T, Tongers J, Wollert KC, Wallentin L. Growth-differentiation factor-15 improves risk stratification in ST-segment elevation myocardial infarction. Eur Heart J. 2007 Dec;28 (23):2858-65
  • Kempf T, Eden M, Strelau J, Naguib M, Willenbockel C, Tongers J, Heineke J, Kotlarz D, Xu J, Molkentin JD, Niessen HW, Drexler H, Wollert KC. The transforming growth factor-beta superfamily member growth-differentiation factor-15 protects the heart from ischemia/reperfusion injury. Circ Res. 2006 Feb 17;98 (3):351-60
  • Kempf T, Horn-Wichmann R, Brabant G, Peter T, Allhoff T, Klein G, Drexler H, Johnston N, Wallentin L, Wollert KC. Circulating concentrations of growth-differentiation factor 15 in apparently healthy elderly individuals and patients with chronic heart failure as assessed by a new immunoradiometric sandwich assay. Clin Chem. 2007 Feb;53 (2):284-91
  • Liu T, Bauskin AR, Zaunders J, Brown DA, Pankhurst S, Russell PJ, Breit SN. Macrophage inhibitory cytokine 1 reduces cell adhesion and induces apoptosis in prostate cancer cells. Cancer Res. 2003 Aug 15;63 (16):5034-40
  • Moore AG, Brown DA, Fairlie WD, Bauskin AR, Brown PK, Munier ML, Russell PK, Salamonsen LA, Wallace EM, Breit SN. The transforming growth factor-ss superfamily cytokine macrophage inhibitory cytokine-1 is present in high concentrations in the serum of pregnant women. J Clin Endocrinol Metab. 2000 Dec;85 (12):4781-8
  • Selander KS, Brown DA, Sequeiros GB, Hunter M, Desmond R, Parpala T, Risteli J, Breit SN, Jukkola-Vuorinen A. Serum macrophage inhibitory cytokine-1 concentrations correlate with the presence of prostate cancer bone metastases. Cancer Epidemiol Biomarkers Pr. 2007 Mar;16 (3):532-7
  • Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, Eling TE, Childs R, Ganz T, Leitman SF, Fucharoen S, Miller JL. High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin. Nat Med. 2007 Sep;13 (9):1096-101
  • Wollert KC, Kempf T, Lagerqvist B, Lindahl B, Olofsson S, Allhoff T, Peter T, Siegbahn A, Venge P, Drexler H, Wallentin L. Growth differentiation factor 15 for risk s
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