GDF-7 belongs to the TGF-β superfamily of growth and differentiation factors. It is expressed selectively by roof plate cells that are located in the developing embryonic central nervous system, and has been shown to influence the neuronal identity of cells within the central nervous system. GDF-7 has also been implicated in the formation, maintenance, and repair of certain cartilage and ligament tissues. Recombinant Human GDF-7 is a 28.0 kDa disulfide-linked homodimer containing two 129 amino acid residues.
Amino Acid Sequence
Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells.
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.