Sandwich ELISA, AChE-labelled antibody
Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Limit of Detection
The Limit of Determination, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 2 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is 10 pg/mL.
Animal studies, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.