Glial derived Neurotrophic Factor Human Recombinant produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2×135 amino acids and having a total molecular mass of 30,360 Dalton. GDNF is purified by proprietary chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. © Analysis by SDS-PAGE.
The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5–10 ng/ml.
GDNF was lyophilized after dialysis against 10 mM sodium citrate and 150 mM NaCl.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution GDNF should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Sterile filtered white lyophilized (freeze-dried) powder.