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Glicentin Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Species:Rat
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RSCYK111R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

30 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.206–50 pmol/ml

Intra-assay (Within-Run)

CV = 6.2%

Inter-assay (Run-to-Run)

CV = 5.4%

Summary

Research topic

Animal studies

Summary

Glicentin is a 69-amino-acid peptide containing glucagon and oxyntomodulin sequences in the molecule. It is
suggested that glicentin and oxyntomodulin are produced in the intestinal L-cells and glucagon in A-cells
in the pancreas, these peptides are derived from a common precursor by two different tissue-specific processing
pathways. In 1983, the amino acid sequence of human glicentin was deduced by Bell et al. from the genomic
sequence of human preproglucagon. Glicentin is a major form of gut glucagon-like immunoreactants (Gut GLIs).
In mammalian small intestine, proglucagon is processed into glicentin, oxyntomodulin, and glucagon -like peptide
1(GLP-1) and glucagon -like peptide 2 (GLP-2). GLP-1(7-37) and GLP-1(7-36)amide have been isolated from
the intestine and pancreas. It has been known that the GLP-1 sequence is well conserved between species in all
mammals studied. Using synthetic peptides, several investigators have demonstrated that in contrast to GLP-1 (1-37), truncated GLP-1(7-36)amide and GLP-1(7-37) have several physiological effects. However, the physiological role of glicentin, a major gut glucagon, is still unclear. It has been known that the circulating level of plasma glicentin-like peptides increases significantly nutrient ingestion. Yanaihara institute Inc. has succeeded in developing a specific and convenient EIA kit for determination of rat glicentin in plasma.

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