Cat # changed from RSCYK090R to YK090
Type
Competitive ELISA, Immobilized antibody
Applications
Plasma
Sample Requirements
50 µl or 100 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
41–10 000 pg/ml
Intra-assay (Within-Run)
3.3-5.1 %
Inter-assay (Run-to-Run)
7.3-18.9 %
Spiking Recovery
101-110 %
Research topic
Diabetology - Other Relevant Products, Animal studies
Summary
According to many studies on glucagon immunoassay, it has been established that the antibody against the C -
terminal fragment ( 19 – 29 ) of glucagon has specific binding with pancreatic glucagon, whereas the antibody
against the N - terminal fragment ( 9 – 19 ) of glucagon has specific binding with both of pancreatic and intestinal
glucagon ( total glucagon ). Once, 30K by Unger et. al had been widely used as an antibody specific for the C -
terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic
glucagon specific antibody using synthetic peptide with the C – terminal fragment ( 19 – 29 ) of glucagon as
immunogen in 1981.
This EIA kit has been developed by using polyclonal antibody against glucagon ( 19 – 29 ), synthetic glucagon as
standard antigen and biotinylated glucagon as labeled antigen for the measurment of rat, mouse or human
glucagon in plasma.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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