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Glucagon Human ELISA (Multispecies specificity)

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Species:Human, Mouse, Rat
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Cat. No. Size Price


RSCYK090R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Plasma

Sample Requirements

50 µl or 100 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

41–10 000 pg/ml

Intra-assay (Within-Run)

3.3-5.1 %

Inter-assay (Run-to-Run)

7.3-18.9 %

Spiking Recovery

101-110 %

Summary

Research topic

Diabetology - Other Relevant Products, Animal studies

Summary

According to many studies on glucagon immunoassay, it has been established that the antibody against the C -
terminal fragment ( 19 – 29 ) of glucagon has specific binding with pancreatic glucagon, whereas the antibody
against the N - terminal fragment ( 9 – 19 ) of glucagon has specific binding with both of pancreatic and intestinal
glucagon ( total glucagon ). Once, 30K by Unger et. al had been widely used as an antibody specific for the C -
terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic
glucagon specific antibody using synthetic peptide with the C – terminal fragment ( 19 – 29 ) of glucagon as
immunogen in 1981.
This EIA kit has been developed by using polyclonal antibody against glucagon ( 19 – 29 ), synthetic glucagon as
standard antigen and biotinylated glucagon as labeled antigen for the measurment of rat, mouse or human
glucagon in plasma.

Product References (4)

References

  • Jaspan JB, Rubenstein AH. Circulating glucagon. Plasma profiles and metabolismin health and disease. Diabetes. 1977 Sep;26(9):887-904. PubMed PMID: 330295. See more on PubMed
  • Nishino T, Kodaira T, Shin S, Imagawa K, Shima K, Kumahara Y, Yanaihara C,Yanaihara N. Glucagon radioimmunoassay with use of antiserum to glucagonC-terminal fragment. Clin Chem. 1981 Oct;27(10):1690-7. PubMed PMID: 6169468. See more on PubMed
  • UNGER RH, EISENTRAUT AM, McCALL MS, KELLER S, LANZ HC, MADISON LL. Glucagonantibodies and their use for immunoassay for glucagon. Proc Soc Exp Biol Med.1959 Oct-Dec;102:621-3. PubMed PMID: 13840405. See more on PubMed
  • UNGER RH, EISENTRAUT AM, McCALL MS, MADISON LL. Glucagon antibodies and animmunoassay for glucagon. J Clin Invest. 1961 Jul;40:1280-9. PubMed PMID:13779204; PubMed Central PMCID: PMC290840. See more on PubMed
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