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Glucagon Human ELISA (Multispecies specificity)

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Species:Human, Mouse, Rat
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Cat. No. Size Price


RSCYK090R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Plasma

Sample Requirements

50 µl or 100 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

41–10 000 pg/ml

Intra-assay (Within-Run)

3.3-5.1 %

Inter-assay (Run-to-Run)

7.3-18.9 %

Spiking Recovery

101-110 %

Summary

Research topic

Diabetology - Other Relevant Products, Animal studies

Summary

According to many studies on glucagon immunoassay, it has been established that the antibody against the C -
terminal fragment ( 19 – 29 ) of glucagon has specific binding with pancreatic glucagon, whereas the antibody
against the N - terminal fragment ( 9 – 19 ) of glucagon has specific binding with both of pancreatic and intestinal
glucagon ( total glucagon ). Once, 30K by Unger et. al had been widely used as an antibody specific for the C -
terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic
glucagon specific antibody using synthetic peptide with the C – terminal fragment ( 19 – 29 ) of glucagon as
immunogen in 1981.
This EIA kit has been developed by using polyclonal antibody against glucagon ( 19 – 29 ), synthetic glucagon as
standard antigen and biotinylated glucagon as labeled antigen for the measurment of rat, mouse or human
glucagon in plasma.

References to Product

References

  • Glucagon related peptides (1993): (Okuno, G., Ohneta, A. and Shima, K.ed.) pp.52-65.Ishiyaku, Tokyo
  • Jaspan, J.B., and Rubenstein, A.H. (1977): Cerculating glucagon:Plasma profiles and matabolism in health and disease. Diabetes., 26:887-902
  • Nishino, T., Kodaira, T., Shin, S., Imagawa, K., Shima, K., Kumahara, Y., Yanaihara, C. and Yanaihara, N.(1981): Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment. Clin . Chem.,27:1690-1697
  • Unger, R.H., Eisentraut, A.M., McCall, M.S., Keller, S., Lanz, H.C. and Madison, L.L. (1959): Glucagon antibodies and their use for immunoassay for glucagon. Proc . Soc . Exp . Biol . Med.,102: 621 - 623
  • Unger, R.H., Eisentraut, A.M., McCall, M.S. and Madison, L.L. (1961): Glucagon antibodies and an immunoassay for glucagon. J . Clin . Invest.,40:1280-1289
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