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Glucagon Human ELISA (Multispecies specificity)

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Species:Human, Mouse, Rat
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Cat. No. Size Price

RSCYK090R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antibody



Sample Requirements

50 µl or 100 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

50–10 000 pg/ml


Research topic

Diabetology - Other Relevant Products


According to many studies on glucagon immunoassay, it has been established that the antibody against the C -
terminal fragment ( 19 – 29 ) of glucagon has specific binding with pancreatic glucagon, whereas the antibody
against the N - terminal fragment ( 9 – 19 ) of glucagon has specific binding with both of pancreatic and intestinal
glucagon ( total glucagon ). Once, 30K by Unger et. al had been widely used as an antibody specific for the C -
terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic
glucagon specific antibody using synthetic peptide with the C – terminal fragment ( 19 – 29 ) of glucagon as
immunogen in 1981.
This EIA kit has been developed by using polyclonal antibody against glucagon ( 19 – 29 ), synthetic glucagon as
standard antigen and biotinylated glucagon as labeled antigen for the measurment of rat, mouse or human
glucagon in plasma.

References to Product


  • Salis ER, Reith DM, Wheeler BJ, Broadbent RS, Medlicott NJ. Insulin resistance, glucagon-like peptide-1 and factors influencing glucose homeostasis in neonates. Arch Dis Child Fetal Neonatal . 2016 Sep 2;
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