Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Growth Differentiation Factor-3 Human E.coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:GDF-3, UNQ222/PRO248
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD172202100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 124 AA. MW: 14.15 kDa (calculated). UniProtKB acc.no. Q9NR23. N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

MKHHHHHHASAAIPVPKLSCKNLCHRHQLFINFRDLGWHKWIIAPKGFMANYCHGECPFSLTISLNSSNYAFMQALMHAVDPEIPQAVCIPTKLSPISMLYQDNNDNVILRHYEDMVVDECGCG

Source

E. coli

SDS-PAGE Gel

14% SDS-PAGE separation of Human GDF-3
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 0.03M Acetate buffer, pH 4

Reconstitution

Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Summary

Research topic

Energy metabolism and body weight regulation

Related Products Documents