Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma
Sample Requirements
50 µl/well
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
20 – 1280 pg/ml
Limit of Detection
5.6 pg/ml
Intra-assay (Within-Run)
n = 8, CV = 3.6%
Inter-assay (Run-to-Run)
n = 6, CV = 5,4%
Spiking Recovery
95,1%
Dilutation Linearity
99,4%
Features
- The total assay time is less than 3.5 hours
- The kit measures H-FABP in human serum, plasma (EDTA, citrate, heparin), urine, cerebrospinal fluid (CSF), bronchoalveolar lavage fluid (BALF) and amniotic fluid
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Cardiovascular disease, Energy metabolism and body weight regulation, Renal disease
Summary
Heart fatty acid binding protein (H-FABP) or FABP3, belongs to the fatty acid binding proteins (FABP) family being characterized by relative tissue specificity. H-FABP is one of the most abundant proteins in the cardio myocytes comprising 5-15% of the total cytosolic protein pool. Except its basic role to transport fatty acids towards the mitochondria for β-oxidation, H-FABP protects against free radical accumulation during myocardial ischemia and influences signal transduction pathways for gene expression via peroxisome proliferator-activated receptor. H-FABP is not totally heart specific and also has been found in skeletal muscle in concentration 10-fold lower than that in heart muscle and in low concentrations in kidney, liver, small intestine, brain, lactating mammary glands, placenta, adipose tissue, adrenal glands and stomach. Under normal conditions H-FABP is presented in plasma at very low concentration. During ischemia, H-FABP leaks out of myocardial tissue and the concentration increases in the blood within 2 hours and is reported to peak at about 4-6 hours and return to normal baseline value in 20 hours. H-FABP is a valuable marker of acute coronary syndromes, heart failure, pulmonary embolism, renal, brain and skeletal muscle injury and its utility increases when is evaluated in combination with other biochemical markers. Thanks to its small size, H-FABP can quickly pass through the kidney to urine, which also gives an opportunity to measure it noninvasively in urine.
