Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Hepatocyte Growth Factor Human (BTI-Tn-5B1-4) Hi-5 Insect cells*

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:(BTI-Tn-5B1-4) Hi-5 Insect cells*
  • Other names:HGF, Hepatopoietin-A, Scatter factor, SF, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, HGF, HPTA
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RBG10177002 2 µg
RBG10177010 10 µg
RBG10177100 100 μg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. Recombinant Human HGF is an 80.0 kDa polypeptide consisting of 697 amino acid residues.

Source

(BTI-Tn-5B1-4) Hi-5 Insect cells*

Purity

98%

Biological Activity

The ED 50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0–40.0 ng/ml.

Endotoxin

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. Do not vortex. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.

Storage/Expiration

 –20°C

Summary

Research topic

Cytokines and chemokines and related molecules

Related Products Docs