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Host Cell Protein-HCP (E.coli) ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
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New RA19015R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Sample Requirements

100 μL/well

Storage/Expiration

Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Range

4.1–3 000.0 ng/mL

Limit of Detection

2.0 ng/mL

Summary

Summary

Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as ‘Host Cell Proteins (HCP),’ are generated and need to be removed. E. coli is commonly used as a production system because it is relatively simple and cost-effective. This kit is intended for use in assessing relative quantities of E. coli HCP in manufactured or research bioproducts.

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