The kit measures hsa-miR-142-5p isolated from human blood
Assay format is 96 wells
Standard is synthetic miRNA-based
Components of the kit are provided ready to use, concentrated or dried
Cardiovascular disease, Oncology, Renal disease
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
The gene encoding hsa-miR-142-5p is located on the human chromosome 17. hsa-miR-142-5p is highly speciﬁc for hematopoietic cells and is overexpressed in pathological conditions such as cancer, immunologically related disorders, small bowel inflammation, renal fibrosis where inflammation occurs and in biopsies from renal transplant patients with acute rejection. It has been suggested that overexpression of hsa-miR-142-5p in patients with chronic antibody-mediated rejection is associated with immunological disorders rather than renal dysfunction. Furthermore, hsa-miR-142-5p may play an onco-miRNA role in progression of renal cell carcinoma.
hsa-miR-142-5p was shown to be downregulated in hepatocellular carcinoma cells and to suppress hepatocelullar carcinoma cell migration. Moreover hsa-miR-142-5p is related to recurrence risk in gastric cancer patients. miRNAs are also implicated in normal heart development and function as well as in cardiac disorders. Downregulation of hsa-miR-142-5p was described in cardiac hypertrophy. Moreover, altered levels of hsa-miR-142-5p in blood were described in dilated cardiomyopathy.
References to Summary
References to miR-142-5p
TSANG, Felice Ho-Ching, et al. MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility. Frontiers of medicine, 2015, 9.3: 331-343.
ZHANG, X., et al. Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection. Annals of oncology, 2011, 22.10: 2257-2266.
LANDGRAF, Pablo, et al. A mammalian microRNA expression atlas based on small RNA library sequencing. Cell, 2007, 129.7: 1401-1414.
DANGER, Richard, et al. Expression of miR-142-5p in peripheral blood mononuclear cells from renal transplant patients with chronic antibody-mediated rejection. PloS one, 2013, 8.4: e60702.
ANGLICHEAU, Dany, et al. MicroRNA expression profiles predictive of human renal allograft status. Proceedings of the National Academy of Sciences, 2009, 106.13: 5330-5335.
LIU, Lingqi, et al. MicroRNA-142-5p promotes cell growth and migration in renal cell carcinoma by targeting BTG3. American journal of translational research, 2017, 9.5: 2394.
SHARMA, Salil, et al. Repression of miR‐142 by p300 and MAPK is required for survival signalling via gp130 during adaptive hypertrophy. EMBO molecular medicine, 2012, 4.7: 617-632.
BARSANTI, Cristina, et al. Differential regulation of microRNAs in end-stage failing hearts is associated with left ventricular assist device unloading. BioMed research international, 2015, 2015.
VOELLENKLE, Christine, et al. MicroRNA signatures in peripheral blood mononuclear cells of chronic heart failure patients. Physiological genomics, 2010, 42.3: 420-426.