miREIA – miRNA enzyme immunoassay
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).
12.5 – 0.39 amol/μl
Limit of Detection
n = 8,
CV = 3.1%
n = 5,
CV = 6.7%
Exact inter-species homology was found for example for:
- It is intended for research use only
- The total assay time is less than 2.5 hours
- The kit measures hsa-miR-145-5p isolated from human blood
- Assay format is 96 wells
- Standard is synthetic miRNA-based
- Components of the kit are provided ready to use, concentrated or dried
Cardiovascular disease, Oncology
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22
nucleotides in length that regulate gene translation through silencing or degradation
of target mRNAs. They are involved in multiple biological processes, including
differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation,
and in pathophysiology of many diseases. Numerous studies have suggested
circulating miRNAs as promising diagnostic and prognostic biomarkers of many
miR-145-5p is considered a member of the p53-tumor suppressor network and has
been found to act as a post-transcriptional regulator of many cancer-related genes.
Deregulations of miR-145-5p were observed in various types of human cancer
including renal cancer, colorectal cancer, bladder cancer, lung cancer, glioblastoma
and prostate cancer.
miR-145-5p is also involved in pathology of cardiovascular disease. miR-145-5p
plasma levels were reported to be significantly lower in patients with acute
myocardial infarction compared to healthy control group. Increased plasma levels
of miR-145-5p were found in population with genetic predisposition to familiar dilated
miR-145-5p is associated also with cerebral ischemia, where expression was
significally higher in ischemic stroke patients when compare to control subjects.