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Manufactured by BioVendor

hsa-miR-150-5p miREIA

  • Regulatory status:RUO
  • Type:miREIA – miRNA enzyme immunoassay
  • Species:Human
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Cat. No. Size Price


New RDM0004H 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Whole blood, PBMC

Sample Requirements

10 µl/well

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

12.5 – 0.39 amol/μl

Limit of Detection

0.13 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 4.9%

Inter-assay (Run-to-Run)

n = 5,
CV = 9.7%

Spiking Recovery

103.5%

Dilutation Linearity

95.9%

Note

Product Manual: miREIA - microRNA enzyme immunoassay

Have you bought miREIA kits and need help with assay procedure? Please look at product manual video how easy using the miREIA method is.


Product Manual: miREIA - microRNA enzyme immunoassay

 

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 2.5 hours
  • The kit measures hsa-miR-150-5p isolated from human blood
  • Assay format is 96 wells
  • Standard is synthetic miRNA-based
  • Components of the kit are provided ready to use, concentrated or dried

Research topic

Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

hsa-miR-150 is encoded by the gene located on the human chromosome 19q13. hsa-miR-150 is selectively expressed in lymph nodes, spleen, and in mature B- and T-cells, and is an important regulator of differentiation and activation of immune cells. It is also involved in the innate and adaptive immune responses. hsa-miR-150 is one of the most studied miRNA and plays an important role in pathogenesis of a number of tumours.

Low levels of hsa-miR-150 in pacient's tumor samples predicted shorter survival and worse response to adjuvant chemotherapy; in contrast, high expression of hsa-miR-150 in pacient's tumor samples indicates better prognosis and better response to adjuvant chemotherapy.

Moreover, over-expression of hsa-miR-150-5p leads to reduced migration and invasion of colorectal carcinoma cells. hsa-miR-150-5p also acts as a metastasis suppressor in colorectal carcinoma.

Plasma levels of hsa-miR-150 were downregulated in patients with acute myeloid leukemia.

hsa-miR-150-5p was upregulated in tumor tissues of non-small-cell lung carcinoma (NSLC) patients and was negatively correlated with expression of the tumor-suppressor geneTP53.

High levels of hsa-miR-150 were observed in gastric cancer tissue. hsa-miR-150 over-expression also promoted gastric cancer proliferation. Furthermore, over-expression of hsa-miR-150 was found in osteosarcoma tumor samples.

References to Summary

References to miR-150-5p

  • XIA, Bairong, et al. Long non-coding RNA ZFAS1 interacts with miR-150-5p to regulate Sp1 expression and ovarian cancer cell malignancy. Oncotarget, 2017, 8.12: 19534.
  • SANG, Wei, et al. MiR-150 impairs inflammatory cytokine production by targeting ARRB-2 after blocking CD28/B7 costimulatory pathway. Immunology letters, 2016, 172: 1-10.
  • LIN, You-Chin, et al. c-Myb is an evolutionary conserved miR-150 target and miR-150/c-Myb interaction is important for embryonic development. Molecular biology and evolution, 2008, 25.10: 2189-2198.
  • ZHOU, Beiyan, et al. miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely. Proceedings of the National Academy of Sciences, 2007, 104.17: 7080-7085.
  • WANG, Wei-Hua, et al. MiR-150-5p suppresses colorectal cancer cell migration and invasion through targeting MUC4. Asian Pac J Cancer Prev, 2014, 15.15: 6269-73.
  • MA, Yanlei, et al. miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer. Gut, 2012, 61.10: 1447-1453.
  • FAYYAD-KAZAN, Hussein, et al. Circulating miR-150 and miR-342 in plasma are novel potential biomarkers for acute myeloid leukemia. Journal of translational medicine, 2013, 11.1: 31.
  • ZHANG, Ni; WEI, Xiang; XU, Lijun. miR‐150 promotes the proliferation of lung cancer cells by targeting P53. FEBS letters, 2013, 587.15: 2346-2351.
  • LULLA, Rishi R., et al. Identification of differentially expressed microRNAs in osteosarcoma. Sarcoma, 2011, 2011.
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