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Manufactured by BioVendor

hsa-miR-191-5p miREIA

  • Regulatory status:RUO
  • Type:miREIA – miRNA enzyme immunoassay
  • Species:Human
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Cat. No. Size Price


New RDM0005H 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Whole blood, PBMC

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

12.5 – 0.39 amol/μl

Limit of Detection

0.13 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 8.1%

Inter-assay (Run-to-Run)

n = 8,
CV = 7.1%

Spiking Recovery

92.0%

Dilutation Linearity

100.0%

Homology

Exact inter-species homology was found for example for:

Bornean orangutan
Brown anole
Carolina anole
Chimpanzee
Chinese hamster
Cow
Horse
King cobra
Mouse
Rabbit
Rat
Wild boar
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 2.5 hours
  • The kit measures hsa-miR-21-5p isolated from human blood
  • Assay format is 96 wells
  • Standard is synthetic miRNA-based
  • Components of the kit are provided ready to use, concentrated or dried

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length, that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miR-191 is expressed as a part of the miR-191/425 cluster on human chromosome 3.

miR-191 is developmentally regulated; significantly higher levels of miR-191 were found in human prefrontal cortex specimens from individuals older than 41 years compared to samples from individuals under 15 years. It was suggested that miR-191-mediated downregulation of a brain-derived neurotrophic factor may contribute to cortical development.

miR-191 was found to be significantly downregulated during terminal erythroid differentiation while its overexpression inhibited erythroid enucleation and chromatin condensation, suggesting that miR-191 plays a crucial role in erythropoiesis. miR-191 was also found to be differentially expressed in monocytes vs. monocyte-derived dendritic cells.

Changes in miR-191 expression have been reported in various malignancies such as breast, colon, lung, liver, prostate, pancreas, stomach, and ovarian cancer, pituitary adenoma, esophageal squamous carcinoma, oral squamous carcinoma, osteosarcoma, bladder, anaplastic large cell lymphoma, acute myeloid leukemia, severe medulloblastoma, retinoblastoma, thyroid follicular tumor, male breast cancer and melanoma.

Besides cancer, aberrant expression and functional abnormalities of miR-191 have been reported in a variety of other diseases, too. miR-191 may be a diagnostic biomarker that could help to distinguish Crohn’s disease from ulcerative colitis. Similarly, miR-191 was shown to be a potential biomarker for pulmonary hypertension, enabling its early detection and also, indicating the disease severity. miR-191 was also found to be induced in nephrotic syndrome in children, which makes it a potential diagnostic marker for this disease.

References to Summary

References to miR-191-5p

  • Kiezun, Adam, et al. "miRviewer: a multispecies microRNA homologous viewer." BMC research notes 5.1 (2012): 92.
  • Griffiths-Jones, Sam, et al. "miRBase: microRNA sequences, targets and gene nomenclature." Nucleic acids research34.suppl_1 (2006): D140-D144.
  • Mellios, Nikolaos, et al. "A set of differentially expressed miRNAs, including miR-30a-5p, act as post-transcriptional inhibitors of BDNF in prefrontal cortex." Human molecular genetics 17.19 (2008): 3030-3042.
  • Zhang, Lingbo, et al. "miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1." Genes & development 25.2 (2011): 119-124.
  • Cekaite, L., T. Clancy, and M. Sioud. "Increased miR-21 expression during human monocyte differentiation into DCs." Frontiers in bioscience (Elite edition) 2 (2010): 818-828.
  • Nagpal, Neha, and Ritu Kulshreshtha. "miR-191: an emerging player in disease biology." Frontiers in genetics 5 (2014): 99.
  • Lin, Jingmei, et al. "MicroRNA expression patterns in indeterminate inflammatory bowel disease." Modern Pathology26.1 (2013): 148.
  • Wei, Chuanyu, et al. "Circulating miRNAs as potential marker for pulmonary hypertension." PloS one 8.5 (2013): e64396.
  • Luo, Yang, et al. "Increased serum and urinary microRNAs in children with idiopathic nephrotic syndrome." Clinical chemistry59.4 (2013): 658-666.
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