Type
miREIA – miRNA enzyme immunoassay
Applications
Whole blood, Cell culture lysates
Sample Requirements
10 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
25 – 0.78 amol/μl
Limit of Detection
0.26 amol/μl
Intra-assay (Within-Run)
n = 8,
CV = 5.2%
Inter-assay (Run-to-Run)
n = 5,
CV = 6.1%
Spiking Recovery
90.0%
Dilutation Linearity
92.3%
Features
- It is intended for research use only
- The total assay time is less than 2.5 hours
- The kit measures hsa-miR-197-3p isolated from human blood
- Assay format is 96 wells
- Standard is synthetic miRNA-based
- Components of the kit are provided ready to use, concentrated or dried
Research topic
Autoimmunity, Cardiovascular disease, Metabolic syndrome, Oncology, Diabetology
Summary
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
miR-197 represents a molecule that is associated with a wide range of pathologic conditions ranging from type 2 diabetes mellitus, myocardial infarction, metabolic syndrome, multiple sclerosis, preeclampsia, hepatitis B virus infection to major human malignancies such as lung cancer, hepatocellular carcinoma, pancreatic cancer, thyroid cancer or cervical carcinoma. miR-197 is distinctively expressed in diffuse large B cell lymphoma and may play a role of a biomarker with potential therapeutic implications in this disease. miR 197 expression was also demonstrated to be considerably downregulated in gastric cancer tissues and cell lines. Its low expression level was associated with increased tumour size, invasive depth, tumour node metastasis staging and lymph node metastasis, whereas high expression of miR 197 inhibited tumour cell proliferation and invasion in vitro.
