hsa-miR-24-3p miREIA

Cat. No.	Size	Price
RDM0012H	96 wells (1 kit)

Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Whole blood, Cell culture lysates

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25 – 0.78 amol/μl

Limit of Detection

0.26 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 6.0%

Inter-assay (Run-to-Run)

n = 5,
CV = 7.7%

Spiking Recovery

106.4%

Dilution Linearity

92.8%

Specificity

Crossreactivity with the miRNA family members exhibiting high sequence identity cannot be excluded.

Note

Product Manual: miREIA - microRNA enzyme immunoassay

Have you bought miREIA kits and need help with assay procedure? Please look at product manual video how easy using the miREIA method is.

Product Manual: miREIA - microRNA enzyme immunoassay

Homology

Exact inter-species homology was found for example for:

Bonobo
Bornean orangutan
Brown anole
Carolina anole
Carp
Catfish
Chimpanzee
Chinese hamster
Cow
Crossostoma
Dog
Fugu
Green spotted puffer

Horse
Japanese rice fish
King cobra
Mouse
Platypus
Rat
Red junglefowl
Western gorilla
Wild boar
Xenopus
Zebra finch
Zebrafish

Summary

Features

It is intended for research use only
The total assay time is less than 2.5 hours
The kit measures hsa-miR-24-3p isolated from human blood
Assay format is 96 wells
Standard is synthetic miRNA-based
Components of the kit are provided ready to use, concentrated or dried

Research topic

Autoimmunity, Cardiovascular disease, Oncology, Neurodegenerative disease

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miR-24 is conserved in various species, and is clustered with miR-23 and miR-27 on the human chromosomes 9 and 19.

miR-24-3p is extensively expressed in various tissues and plays an important role in various physiological or pathological conditions, such as oral cancers, prostate cancer, hyperglycemia, multiple sclerosis or Alzheimer's disease. Expression of miR-24-3p was reported to be elevated in breast cancer patients with metastasis, both in plasma and breast cancer tissue.

miR-24-3p expression level was significantly increased in human as well as rat cardiac tissue after geart failure. In heart failure animal models, abnormally high expression of miR-24 led to decreased efficiency of excitation-contraction. miR-24-3p is a key mediator promoting cardiac hypertrophy; one of the potential mechanisms is driving cells in G0/G1 phase into S phase.

Summary References (11)

References to miR-24-3p

Lin, Shu-Chun, et al. "miR-24 up-regulation in oral carcinoma: positive association from clinical and in vitro analysis." Oral oncology 46.3 (2010): 204-208.
Landgraf, Pablo, et al. "A mammalian microRNA expression atlas based on small RNA library sequencing." Cell 129.7 (2007): 1401-1414.
Liu, Xiqiang, et al. "MicroRNA‐24 targeting RNA‐binding protein DND1 in tongue squamous cell carcinoma." FEBS letters 584.18 (2010): 4115-4120.
Lynch, Seodhna M., et al. "miR‐24 regulates CDKN1B/p27 expression in prostate cancer." The Prostate 76.7 (2016): 637-648.
Xiang, Yaozu, et al. "Hyperglycemia repression of miR-24 coordinately upregulates endothelial cell expression and secretion of von Willebrand factor." Blood (2015): blood-2015.
Vistbakka, J., et al. "Evaluation of serum miR‐191‐5p, miR‐24‐3p, miR‐128‐3p, and miR‐376c‐3 in multiple sclerosis patients." Acta Neurologica Scandinavica (2018).
Müller, Mareike, et al. "MicroRNAs in Alzheimer's disease: differential expression in hippocampus and cell-free cerebrospinal fluid." Neurobiology of aging 35.1 (2014): 152-158.
Khodadadi-Jamayran, Alireza, et al. "Prognostic role of elevated mir-24-3p in breast cancer and its association with the metastatic process." Oncotarget 9.16 (2018): 12868.
Van Rooij, Eva, et al. "A signature pattern of stress-responsive microRNAs that can evoke cardiac hypertrophy and heart failure." Proceedings of the National Academy of Sciences103.48 (2006): 18255-18260.
Haeusler, Aaron R., et al. "C9orf72 nucleotide repeat structures initiate molecular cascades of disease." Nature 507.7491 (2014): 195.
Gao, Juan, et al. "Cardiac Hypertrophy is Positively Regulated by MicroRNA-24 in Rats." Chinese medical journal 131.11 (2018): 1333

Docs

Product Datasheet (RUO)

Datasheet PDF (RUO)

Safety Information (RUO)

MSDS (RUO)

Product Brochure

miRNA quantification