Type
miREIA – miRNA enzyme immunoassay
Applications
Whole blood, Cell culture lysates
Sample Requirements
10 µl/well
Storage/Expiration
Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
25 – 0.78 amol/μl
Limit of Detection
0.26 amol/μl
Intra-assay (Within-Run)
n = 8,
CV = 6.0%
Inter-assay (Run-to-Run)
n = 5,
CV = 7.7%
Spiking Recovery
106.4%
Dilutation Linearity
92.8%
Homology
Exact inter-species homology was found for example for:
Bonobo
Bornean orangutan
Brown anole
Carolina anole
Carp
Catfish
Chimpanzee
Chinese hamster
Cow
Crossostoma
Dog
Fugu
Green spotted puffer
Horse
Japanese rice fish
King cobra
Mouse
Platypus
Rat
Red junglefowl
Western gorilla
Wild boar
Xenopus
Zebra finch
Zebrafish
Features
- It is intended for research use only
- The total assay time is less than 2.5 hours
- The kit measures hsa-miR-24-3p isolated from human blood
- Assay format is 96 wells
- Standard is synthetic miRNA-based
- Components of the kit are provided ready to use, concentrated or dried
Research topic
Autoimmunity, Cardiovascular disease, Oncology, Neurodegenerative disease
Summary
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
miR-24 is conserved in various species, and is clustered with miR-23 and miR-27 on the human chromosomes 9 and 19.
miR-24-3p is extensively expressed in various tissues and plays an important role in various physiological or pathological conditions, such as oral cancers, prostate cancer, hyperglycemia, multiple sclerosis or Alzheimer's disease. Expression of miR-24-3p was reported to be elevated in breast cancer patients with metastasis, both in plasma and breast cancer tissue.
miR-24-3p expression level was significantly increased in human as well as rat cardiac tissue after geart failure. In heart failure animal models, abnormally high expression of miR-24 led to decreased efficiency of excitation-contraction. miR-24-3p is a key mediator promoting cardiac hypertrophy; one of the potential mechanisms is driving cells in G0/G1 phase into S phase.
