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Manufactured by BioVendor

hsa-miR-34a-5p miREIA

  • Regulatory status:RUO
  • Type:miREIA – miRNA enzyme immunoassay
  • Species:Human
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Cat. No. Size Price


New RDM0051H 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Tissue extract, Cell culture lysate

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25.0 – 0.78 amol/μl

Limit of Detection

0.26 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 10.4%

Inter-assay (Run-to-Run)

n = 5,
CV = 10.8%

Spiking Recovery

97.4%

Dilution Linearity

105.9%

Specificity

Crossreactivity with the miRNA family members exhibiting high sequence identity cannot be excluded.

Note

Product Manual: miREIA - microRNA enzyme immunoassay

Have you bought miREIA kits and need help with assay procedure? Please look at product manual video how easy using the miREIA method is.


Product Manual: miREIA - microRNA enzyme immunoassay

 

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 2.5 hours
  • The kit measures hsa-miR-34a-5p isolated from human blood
  • Assay format is 96 wells
  • Standard is synthetic miRNA-based
  • Components of the kit are provided ready to use, concentrated or dried

Research topic

Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miR-34a-5p is one of the most characterized tumor suppressor miRNAs in a variety of tumors and it plays a crucial role in regulating cancer-related processes, such as cell proliferation, apoptosis, epithelial-mesenchymal transition and metastasis. miR-34a-5p is downregulated in many tumours where it inhibits cell invasion and migration.

Primary colorectal cancer (CRC) tissues from patients showed lower miR-34a-5p expression when compared with matched non-tumour tissues. The expression level of miR-34a-5p in patients with p53-positive expression was higher than that in patients with p53-negative expression which indicates that the growth-inhibiting effect by miR-34a-5p was dependent on p53.

Expression of miR-34a was up-regulated in cancer tissue compared to adjacent tissue. High miR-34a expression was associated with better survival of patients. miR-34a showed lower expression levels in male patients with lymph node involvement, and a trend towards decreased expression in male patients with distant metastases. Male patients, but not female patients, with high expression of miR-34a and who were free of distant metastases and/or lymph node involvement showed better survival. miR-34a-5p was downregulated while lymphoid enhancer-binding factor 1 (LEF1) was upregulated in esophageal squamous cell carcinoma (ESCC) tissue and cell lines. Overexpression of miR-34a-5p could inhibit proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of ESCC cells.

Apigenin induces apoptosis through down-regulation of a zinc-finger transcription factor (SNAI1) by up-regulating miR-34a-5p in lung cancer cells.

Summary References (3)

References to miR-34a-5p

  • Aida R, Hagiwara K, Okano K, Nakata K, Obata Y, Yamashita T, Yoshida K, Hagiwara H. miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells. Mol Biol Rep. 2021 Mar;48(3):2291-2297. doi: 10.1007/s11033-021-06255-7. Epub 2021 Mar 6. PMID: 33675467; PMCID: PMC8060201. See more on PubMed
  • Hasakova K, Reis R, Vician M, Zeman M, Herichova I. Expression of miR-34a-5p is up-regulated in human colorectal cancer and correlates with survival and clock gene PER2 expression. PLoS One. 2019 Oct 28;14(10):e0224396. doi: 10.1371/journal.pone.0224396. PMID: 31658284; PMCID: PMC6816564. See more on PubMed
  • Wang X, Zhao Y, Lu Q, Fei X, Lu C, Li C, Chen H. MiR-34a-5p Inhibits Proliferation, Migration, Invasion and Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma by Targeting LEF1 and Inactivation of the Hippo-YAP1/TAZ Signaling Pathway. J Cancer. 2020 Mar 4;11(10):3072-3081. doi: 10.7150/jca.39861. PMID: 32226522; PMCID: PMC7086260. See more on PubMed
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