The kit measures hsa-miR-5100 isolated from human blood
Assay format is 96 wells
Standard is synthetic miRNA-based
Components of the kit are provided ready to use, concentrated or dried
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
miR-5100 contributes to human carcinogenesis in various cancers, including glioblastoma, hepatocellular carcinoma (HCC), lung cancer and oral squamous cell carcinoma (OSCC). It was observed that miR-5100 was overexpressed in a subset of lung cancer tissues. miR-5100 overexpression increases the cisplatin resistance of the lung cancer stem cells (LCSCs) through the mitochondrial apoptosis pathway. The relative level of miR-5100 expression in non-small-cell lung cancer (NSCLC) tissues was significantly upregulated, compared with that in corresponding noncancerous tissues. NSCLC patients with higher miR-5100 expression had significantly poorer overall survival and progression-free survival. Overexpression of miR-5100 also inhibited colony formation, cell migration and invasion of pancreatic cancer cells.
miR-5100 plays an important role in the idiopathic pulmonary fibrosis (IPF). The study indicated that miR-5100 promotes the proliferation and metastasis of lung epithelial cells.
The expression levels of let-7f-5p, miR-5100 and miR-25-3p were significantly associated with the incidence rate of childhood acute lymphocytic leukemia (cALL), and these miRNAs might serve as promising biomarkers for cALL.
It was also shown, that miR-5100 negatively regulates Tob2, which acts as a negative regulator of osteogenesis. The important bone-related transcription factor osterix, which can be degraded by binding to Tob2, was influenced by miR-5100 during osteoblast differentiation.
The expression of miR-371b-5p and miR-5100 was increased significantly in the serum of systemic lupus erythematosus (SLE), compared with healthy controls and rheumatoid arthritis (RA) group. It was also higher in active SLE than in inactive SLE. The association was found between the expression levels of miR-371b-5p and miR-5100 and clinical parameters of SLE.
Summary References (12)
References to miR-5100
Dong L, Li Y, Han C, Wang X, She L, Zhang H. miRNA microarray reveals specific expression in the peripheral blood of glioblastoma patients. Int J Oncol. 2014; 45: 746–56.
Sun X, Wang M, Liu H, Wang J. MicroRNA-423 enhances the invasiveness of hepatocellular carcinoma via regulation of BRMS1. Am J Transl Res. 2017; 9: 5576–84.
Huang H, Jiang Y, Wang Y, Chen T, Yang L, He H. et al. miR-5100 promotes tumor growth in lung cancer by targeting Rab6. Cancer Lett. 2015; 362: 15–24.
Wang T, Liu X, Tian Q, Liang T, Chang P: Increasing expression of miR-5100 in non-small-cell lung cancer and correlation with prognosis. Eur Rev Med Pharmacol Sci. 2017 Aug;21(16):3592-3597.
Shi J, Bao X, Liu Z, Zhang Z, Chen W, Xu Q: Serum miR-626 and miR-5100 are Promising Prognosis Predictors for Oral Squamous Cell Carcinoma. Theranostics. 2019 Jan 25;9(4):920-931.
WANg y, CheN J, LiN z, CAo J, huANg h, JiANg y, heh, yANg L, reN N, Liu g. Role of deregulated mi-croRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed, paraffin-em-bedded samples. Oncol Lett 2016; 11: 801-808
Yang L et al.: MiR-5100 increases the cisplatin resistance of the lung cancer stem cells by inhibiting the Rab6. Mol Carcinog. 2018 Mar;57(3):419-428.
Chijiiwa Y et al.: Overexpression of microRNA-5100 decreases the aggressive phenotype of pancreatic cancer cells by targeting PODXL. Int J Oncol. 2016 Apr;48(4):1688-700.
Li CY et al.: MiR-5100 targets TOB2 to drive epithelial-mesenchymal transition associated with activating smad2/3 in lung epithelial cells. Am J Transl Res. 2017 Oct 15;9(10):4694-4706.
Yuan DM et al.: Association between expression of plasma miRNA and the risk of childhood acute lymphocytic leukemia. Zhonghua Liu Xing Bing Xue Za Zhi. 2017 Sep 10;38(9):1252-1258.
Wang H et al.: MiR-5100 promotes osteogenic differentiation by targeting Tob2. J Bone Miner Metab. 2017 Nov;35(6):608-615.
Zeng L et al.: Serum miRNA-371b-5p and miRNA-5100 act as biomarkers for systemic lupus erythematosus. Clin Immunol. 2018 Nov;196:103-109.