Select country set
language
Menu Shopping cart 0,00 Search
Manufactured by BioVendor

hsa-miR-941 miREIA

  • Regulatory status:RUO
  • Type:miREIA – miRNA enzyme immunoassay
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


New RDM0053H 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Tissue extract, Whole blood

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25.0 – 0.39 amol/μl

Limit of Detection

0.13 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 7.4%

Inter-assay (Run-to-Run)

n = 5,
CV = 10.2%

Spiking Recovery

97.3%

Dilution Linearity

104.6%

Specificity

Crossreactivity with the miRNA family members exhibiting high sequence identity cannot be excluded.

Note

Product Manual: miREIA - microRNA enzyme immunoassay

Have you bought miREIA kits and need help with assay procedure? Please look at product manual video how easy using the miREIA method is.


Product Manual: miREIA - microRNA enzyme immunoassay

 

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 2.5 hours
  • The kit measures hsa-miR-941 isolated from human blood
  • Assay format is 96 wells
  • Standard is synthetic miRNA-based
  • Components of the kit are provided ready to use, concentrated or dried

Research topic

Cardiovascular disease, Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

The miR-941 gene is only found in humans where it first appeared between one and six million years ago. miR-941 is highly expressed in pluripotent cells, repressed upon differentiation and preferentially targets genes in hedgehog- and insulin-signalling pathways, thus suggesting roles in cellular differentiation. Human-specific effects of miR-941 regulation are detectable in the brain and affect genes involved in neurotransmitter signalling. Taken together, these results implicate miR-941 in human evolution, and provide an example of rapid regulatory evolution in the human lineage.

miR-941 was significantly down-regulated in hepatocellular carcinoma (HCC) tissues and cell lines and was generally hypermethylated in HCC. The overexpression of miR-941 suppressed in vitro cell proliferation, migration, and invasion and inhibited the metastasis of HCC cells in vivo.

miR-941 expression was relatively higher in patients with acute coronary syndrome (ACS) and ST-segment elevation myocardial infarction (STEMI). Plasma miR-941 level was elevated in patients with NSTE-ACS or STEMI as compared with that in patients without coronary artery disease.

Summary References (3)

References to miR-941

  • Bai R, Yang Q, Xi R, Li L, Shi D, Chen K. miR-941 as a promising biomarker for acute coronary syndrome. BMC Cardiovasc Disord. 2017 Aug 22;17(1):227. doi: 10.1186/s12872-017-0653-8. PMID: 28830367; PMCID: PMC5568367. See more on PubMed
  • Hu HY, He L, Fominykh K, Yan Z, Guo S, Zhang X, Taylor MS, Tang L, Li J, Liu J, Wang W, Yu H, Khaitovich P. Evolution of the human-specific microRNA miR-941. Nat Commun. 2012;3:1145. doi: 10.1038/ncomms2146. PMID: 23093182; PMCID: PMC3493648. See more on PubMed
  • Zhang PP, Wang XL, Zhao W, Qi B, Yang Q, Wan HY, Shuang ZY, Liu M, Li X, Li S, Tang H. DNA methylation-mediated repression of miR-941 enhances lysine (K)-specific demethylase 6B expression in hepatoma cells. J Biol Chem. 2014 Aug 29;289(35):24724-35. doi: 10.1074/jbc.M114.567818. Epub 2014 Jul 21. PMID: 25049231; PMCID: PMC4148894. See more on PubMed
Related Products Docs