The kit measures hsa-miR-941 isolated from human blood
Assay format is 96 wells
Standard is synthetic miRNA-based
Components of the kit are provided ready to use, concentrated or dried
Cardiovascular disease, Oncology
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
The miR-941 gene is only found in humans where it first appeared between one and six million years ago. miR-941 is highly expressed in pluripotent cells, repressed upon differentiation and preferentially targets genes in hedgehog- and insulin-signalling pathways, thus suggesting roles in cellular differentiation. Human-specific effects of miR-941 regulation are detectable in the brain and affect genes involved in neurotransmitter signalling. Taken together, these results implicate miR-941 in human evolution, and provide an example of rapid regulatory evolution in the human lineage.
miR-941 was significantly down-regulated in hepatocellular carcinoma (HCC) tissues and cell lines and was generally hypermethylated in HCC. The overexpression of miR-941 suppressed in vitro cell proliferation, migration, and invasion and inhibited the metastasis of HCC cells in vivo.
miR-941 expression was relatively higher in patients with acute coronary syndrome (ACS) and ST-segment elevation myocardial infarction (STEMI). Plasma miR-941 level was elevated in patients with NSTE-ACS or STEMI as compared with that in patients without coronary artery disease.
Summary References (3)
References to miR-941
Bai R, Yang Q, Xi R, Li L, Shi D, Chen K. miR-941 as a promising biomarker
for acute coronary syndrome. BMC Cardiovasc Disord. 2017 Aug 22;17(1):227. doi:
10.1186/s12872-017-0653-8. PMID: 28830367; PMCID: PMC5568367. See more on PubMed
Hu HY, He L, Fominykh K, Yan Z, Guo S, Zhang X, Taylor MS, Tang L, Li J, Liu
J, Wang W, Yu H, Khaitovich P. Evolution of the human-specific microRNA miR-941.
Nat Commun. 2012;3:1145. doi: 10.1038/ncomms2146. PMID: 23093182; PMCID:
PMC3493648. See more on PubMed
Zhang PP, Wang XL, Zhao W, Qi B, Yang Q, Wan HY, Shuang ZY, Liu M, Li X, Li
S, Tang H. DNA methylation-mediated repression of miR-941 enhances lysine
(K)-specific demethylase 6B expression in hepatoma cells. J Biol Chem. 2014 Aug
29;289(35):24724-35. doi: 10.1074/jbc.M114.567818. Epub 2014 Jul 21. PMID:
25049231; PMCID: PMC4148894. See more on PubMed