The kit measures hsa-miR-let-7a-5p isolated from human blood
Assay format is 96 wells
Standard is synthetic miRNA-based
Components of the kit are provided ready to use, concentrated or dried
Immune Response, Infection and Inflammation, Oncology
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
The let-7 miRNA family consists of 11 closely related genes. The gene for let-7a is located on the chromosome 22q13.31 and is associated with CpG islands. Let-7a-5p is a microRNA, which inhibits migration, invasion, as well as epithelial-mesenchymal transition by targeting several oncogenes. Moreover, cell proliferation, colony formation, migration and invasion were decreased after induced overexpression of let-7a in breast cancer cells. Deregulation of miR-let-7a-5p is associated with oncological diseases. Thus, miR-let-7a acts as a tumor suppressor and is down-regulated in many types of cancer. Reduced levels of miR-let-7a-5p were reported in lung squamous carcinoma, colorectal cancer, urothelial tumors, lymphoms, breast cancer and many other diagnoses.
Apart from its role in tumor suppression, involvement of miR-let-7a, in cell proliferation pathway in human cells has been reported, and its connection to cerebrovascular and neurodegenerative diseases has been observed. miRNA-let-7a has been shown to directly alter cell cycle progression and proinflammatory cytokine production in brain. In response to inflammation, miR-let-7a participates in reduction of nitrite production and expression of inducible nitric oxide synthase (iNOS) and interleukin-6 and is involved in increased expression of brain-derived inflammatory cytokines in microglia. Thus, miRNA-let-7a could act as a regulator of the function of microglia in inflammation.
Summary References (10)
References to miR-let-7a-5p
REINHART, Brenda J., et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. nature, 2000, 403.6772: 901.
BRUECKNER, Bodo, et al. The human let-7a-3 locus contains an epigenetically regulated microRNA gene with oncogenic function. Cancer research, 2007, 67.4: 1419-1423.
TSANG, Wing Pui; KWOK, Tim Tak. Let-7a microRNA suppresses therapeutics-induced cancer cell death by targeting caspase-3. Apoptosis, 2008, 13.10: 1215-1222.
LIU, Tsang-Pai, et al. Down-regulation of let-7a-5p predicts lymph node metastasis and prognosis in colorectal cancer: Implications for chemotherapy. Surgical oncology, 2016, 25.4: 429-434.
TAO, Jun, et al. Identification of circulating microRNA signatures for upper tract urothelial carcinoma detection. Molecular medicine reports, 2015, 12.5: 6752-6760.
SAMPSON, Valerie B., et al. MicroRNA let-7a down-regulates MYC and reverts MYC-induced growth in Burkitt lymphoma cells. Cancer research, 2007, 67.20: 9762-9770.
LIU, Kui, et al. Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1. International journal of oncology, 2015, 46.6: 2526-2534.
JOHNSON, Charles D., et al. The let-7 microRNA represses cell proliferation pathways in human cells. Cancer research, 2007, 67.16: 7713-7722.
CHO, Kyoung Joo, et al. MicroRNA-Let-7a regulates the function of microglia in inflammation. Molecular and Cellular Neuroscience, 2015, 68: 167-176.
CHAFIN, Cristen B., et al. MicroRNA-let-7a promotes E2F-mediated cell proliferation and NFκB activation in vitro. Cellular & molecular immunology, 2014, 11.1: 79.