Type
miREIA – miRNA enzyme immunoassay
Applications
Whole blood, Cell culture lysates
Sample Requirements
10 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
40 – 1.25 amol/μl
Limit of Detection
0.42 amol/μl
Intra-assay (Within-Run)
n = 8,
CV = 5.3%
Inter-assay (Run-to-Run)
n = 5,
CV = 8.7%
Spiking Recovery
94.7%
Dilutation Linearity
102.1%
Features
- It is intended for research use only
- The total assay time is less than 2.5 hours
- The kit measures hsa-miR-let-7a-5p isolated from human blood
- Assay format is 96 wells
- Standard is synthetic miRNA-based
- Components of the kit are provided ready to use, concentrated or dried
Research topic
Oncology, Inflammation
Summary
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
The let-7 miRNA family consists of 11 closely related genes. The gene for let-7a is located on the chromosome 22q13.31 and is associated with CpG islands. Let-7a-5p is a microRNA, which inhibits migration, invasion, as well as epithelial-mesenchymal transition by targeting several oncogenes. Moreover, cell proliferation, colony formation, migration and invasion were decreased after induced overexpression of let-7a in breast cancer cells. Deregulation of miR-let-7a-5p is associated with oncological diseases. Thus, miR-let-7a acts as a tumor suppressor and is down-regulated in many types of cancer. Reduced levels of miR-let-7a-5p were reported in lung squamous carcinoma, colorectal cancer, urothelial tumors, lymphoms, breast cancer and many other diagnoses.
Apart from its role in tumor suppression, involvement of miR-let-7a, in cell proliferation pathway in human cells has been reported, and its connection to cerebrovascular and neurodegenerative diseases has been observed. miRNA-let-7a has been shown to directly alter cell cycle progression and proinflammatory cytokine production in brain. In response to inflammation, miR-let-7a participates in reduction of nitrite production and expression of inducible nitric oxide synthase (iNOS) and interleukin-6 and is involved in increased expression of brain-derived inflammatory cytokines in microglia. Thus, miRNA-let-7a could act as a regulator of the function of microglia in inflammation.
