Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Tissue extract, Cell lysate
Storage/Expiration
2–8°C
Calibration Curve
Calibration Range
150–2.34 ng/mL
Limit of Detection
1.54 ng/mL
Features
- ELISA kit used to quantitate Hsc70 concentration in samples
- The total assay time is less than 4 hours
Research topic
Others
Summary
Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments
of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and
the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at
least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the
C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity
which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When
hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding
activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide
binding capacity. Polypeptide binding ability therefore resided within the C-terminal half
(4). The structure of this ATP binding domain displays multiple features of nucleotide
binding proteins (5).
When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70
family, hsp 70 (hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence
identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly
inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is
regulated by ATP. These two heat shock proteins move together in the cell experiencing
stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the
recovery of centrosomal structure and function after heat shock (6).
