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hsCRP Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:C-Reactive Protein
  • Species:Mouse
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Cat. No. Size Price


RH971CRP01MR 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5µl/well

Storage/Expiration

C-reactive protein kit components should be stored at 2–8°C. Bring them to room temperature (20–25°C) before opening bottles and plate pouches. Diluted conjugate remaining after use should be discarded. TMB substrate and stop solution are also stable at room temperature.

Calibration Curve

Calibration Range

1.56–25.0 ng/ml

Limit of Detection

2.5 ng/ml

Intra-assay (Within-Run)

n = 12; CV = 4%

Inter-assay (Run-to-Run)

n = 12; CV = 6%

Summary

Research topic

Animal studies, Immune Response, Infection and Inflammation, Sepsis

Summary

CRP is an acute-phase protein, produced exclusively in the liver. Serum concentration of CRP increases significantly in cases of both infectious and noninfectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours. CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatic arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease. The CRP level increases dramatically following microbial infections, and this can be particularly helpful for the diagnosis and monitoring of bacterial septicemia in neonates and other immunocompromised patients at risk.

References to Summary

References to CRP

  • Gewurz H, Mold C, Siegel J, Fiedel B. C-reactive protein and the acute phase response. Adv Intern Med. 1982;27:345-72
  • Helgeson NGP, Adamson DM, Pike RB, James DS, Nicodemus DS, Lee BA, Miller GW. C-Reactive Protein : Laboratory Medicine, Vol. 2 (Race G. J., Ed.). Harper & Row, Hagerstown, chap. 1973;
  • Johnson HL, Chiou CC, Cho CT. Applications of acute phase reactants in infectious diseases. J Microbiol Immunol Infect. 1999 Jun;32 (2):73-82
  • Powell LJ. C-reactive protein--a review. Am J Med Technol. 1979 Feb;45 (2):138-42
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