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Hsp65 StressXpress® Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Hsp65
  • Species:Human
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Cat. No. Size Price


RSKT-113-480R 5x 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Tissue extract, Cell lysate

Storage/Expiration

2–8°C

Calibration Curve

Calibration Range

0.344–22 ng/mL

Limit of Detection

0.06 ng/mL

Summary

Features

  • ELISA kit used to quantitate Hsp65 concentration in samples
  • The total assay time is less than 4 hours

Research topic

Others

Summary

Hsp65 isolated from Mycobacterium bovis BCG, is a member of the hsp60 family of heat shock proteins (2,3). Hsp60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, Hsp60 functions as a chaperonin to assist in folding linear amino acid chains into their respective threedimensional structure. Hsp60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically,sequence comparison of Hsp65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG Hsp65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6

References to Summary

References to Heat Shock 65kDa Protein

  • Cobelens PM, Kavelaars A, van der Zee R, van Eden W, Heijnen CJ. Dynamics of mycobacterial HSP65-induced T-cell cytokine expression during oral tolerance induction in adjuvant arthritis. Rheumatology (Oxford). 2002 Jul;41 (7):775-9
  • Koll H, Guiard B, Rassow J, Ostermann J, Horwich AL, Neupert W, Hartl FU. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell. 1992 Mar 20;68 (6):1163-75
  • Shinnick TM, Sweetser D, Thole J, van Embden J, Young RA. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect Immun. 1987 Aug;55 (8):1932-5
  • Thole JE, Dauwerse HG, Das PK, Groothuis DG, Schouls LM, van Embden JD. Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli. Infect Immun. 1985 Dec;50 (3):800-6
  • Thole JE, Keulen WJ, De Bruyn J, Kolk AH, Groothuis DG, Berwald LG, Tiesjema RH, van Embden JD. Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12. Infect Immun. 1987 Jun;55 (6):1466-75
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