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Human Brain-derived Neurotrophic Factor (BDNF) ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:BDNF; Abrineurin; Neurotrophin; ANON2; BULN2
  • Species:Human
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New RBG19001R
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

100 µL/well

Storage/Expiration

Store at 4°C upon arrival for 6 months or -20°C for 12 months.

Calibration Range

31.2pg/ml-2000pg/ml

Limit of Detection

<15 pg/ml

Summary

Features

  • Intended for Research Use only
  • The assay time is less than 5 hours.
  • The kit measures BDNF in serum, plasma, cell culture supernatants.
  • Assay format is 96 wells -Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Diabetology - Autoimmunity, Energy metabolism and body weight regulation, Neural tissue markers

Summary

BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues.

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