Type
Sandwich ELISA
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant
Storage/Expiration
2–8°C
Calibration Range
31,25-1000 pg/ml
Intra-assay (Within-Run)
CV - 1,4%
Inter-assay (Run-to-Run)
CV - 2,76%
Spiking Recovery
96%
Research topic
Autoimmunity, Immunology, Oncology
Summary
Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response.
The receptor complex mediating the biological activities consists of two distinct membrane-bound glycoproteins:
- IL-6 Receptor (IL-6R, gp80) is 80 kDa alpha subunit, transmembrane glycoprotein that is 449 aa long. It binds IL-6 with low affinity
- A signal-transducing non-ligand binding 130 kDa component (gp130) that is required for high-affinity binding complex (Kd = 50 pM)
Soluble IL-6 receptor (sIL-6R) is a 50-55 kDa ligand binding protein, derived from the extracellular part of the gp80 receptor by either proteolytic cleavage or by alternative splicing. The soluble form of IL-6R can bind its ligand and induce cellular responses by association with gp130, thus acting as an IL-6 agonist.
Dysregulated production of IL6 and its receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Elevated IL-6 concentrations have been reported in numerous clinical disorders where they appear to orchestrate a variety of inflammatory responses.
