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Manufactured by BioVendor

Hyaluronidase-1 (HYAL1) Human HEK293 Recombinant

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293 cells
  • Other names:Hyaluronidase-1, HYAL-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1, HYAL1, Hyaluronidase 1, Hyaluronoglucosaminidase 1, Hyaluronoglucosaminidase1, LUCA 1, MPS9, NAT6, Plasma hyaluronidase, Tumor suppressor LUCA 1
  • Species:Human
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Cat. No. Size Price


New RP1791155010 10 μg
New RP17911551MG 1 mg
Product Details
Technical Data

Type

Recombinant protein

Description

HYAL1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (22-435 a.a) containing a total of 420 amino acids, having a molecular mass of 46.9 kDa. HYAL1 is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.

Amino Acid Sequence

FRGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMWHHHHHH

Source

HEK293 cells

Purity

Greater than 90.0%

Formulation

The HYAL1 solution (0.25mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).

Applications

Cell culture and/or animal studies, In vitro

Shipping

On frozen gel ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Note

This BioVendor product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Summary

Summary

Human hyaluronidase-1 (Hyal-1) is a 435 amino acid residue protein exhibiting a narrow acidic pH activity (3−4.5), consistent with the low pH in the lysosome. The protein contains 10 cysteines, three predicted N-glycosylation sites, and an N-terminal endoplasmic reticulum signal sequence. It cleaves hyaluronic acid (HA) substrates of all sizes into short chains, down to tetrasaccharides.

Human Hyal-1 is a lysosomal enzyme responsible for the hydrolysis of intracellular HA and is also detected in the plasma and in urine. Increased expression of HYAL1 mRNA and higher levels of hHyal-1 are documented in many diseases, and in bladder and prostate cancers. The enzyme is responsible for the production of angiogenic HA fragments. Because of its presence in urine and change in expression level in bladder and prostate cancers, the use of hHyal-1 as a marker of these diseases has been considered. The role of hHyal-1 in tumor progression depends on its cellular concentration; it functions as a tumor suppressor at low or very high concentration and as a tumor promoter at moderate concentration.

Inactivating mutations of hHyal-1 are linked to the human genetic disorder mucopolysaccharidosis IX (also known as hyaluronidase deficiency), characterized by diminished stature, periarticular soft tissue masses, and absence of neurological and visceral involvement. The plasma HA concentration of patients with this disorder is 40−90-fold higher than in normal serum.

Summary References (0)

References to

17503783, 32363767, 32432341, 31758257, 30321638

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